Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.12 extracted from

  • Dagher, S.M.; Deal, W.C.
    Pig liver glyceraldehyde-3-P dehydrogenase: purification, crystallization, and characterization (1977), Arch. Biochem. Biophys., 179, 643-653.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Sus scrofa

General Stability

General Stability Organism
5 M, 0°C, 1 h, complete loss of activity, 5 mM NAD+ protects Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
ATP at 0°C, loss of activity. Some of the lost activity is regained upon warming to room temperature Sus scrofa
NAD+
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
NAD+
-
Sus scrofa
0.023
-
NADH
-
Sus scrofa
0.24
-
D-glyceraldehyde 3-phosphate
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
4 * 38000, SDS-PAGE Sus scrofa
148000
-
equilibrium sedimentation Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Sus scrofa 3-phospho-D-glyceroyl phosphate + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 38000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.8
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
7.4 9.6 about 50% of maximal activity at pH 7.4 and at ph 9.6 Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+ 2-3 mol of tightly bound NAD+ Sus scrofa