Crystallization (Comment) | Organism |
---|---|
- |
Sus scrofa |
General Stability | Organism |
---|---|
5 M, 0°C, 1 h, complete loss of activity, 5 mM NAD+ protects | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | at 0°C, loss of activity. Some of the lost activity is regained upon warming to room temperature | Sus scrofa | |
NAD+ | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
NAD+ | - |
Sus scrofa | |
0.023 | - |
NADH | - |
Sus scrofa | |
0.24 | - |
D-glyceraldehyde 3-phosphate | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, SDS-PAGE | Sus scrofa |
148000 | - |
equilibrium sedimentation | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Sus scrofa | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000, SDS-PAGE | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.8 | - |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9.6 | about 50% of maximal activity at pH 7.4 and at ph 9.6 | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | 2-3 mol of tightly bound NAD+ | Sus scrofa |