Protein Variants | Comment | Organism |
---|---|---|
D186G | behavior in NAD+ binding is similar to that of the wild type enzyme | Geobacillus stearothermophilus |
D186G/E276G | positive cooperativity in binding the coenzyme NAD+ | Geobacillus stearothermophilus |
E276G | behavior in NAD+ binding is similar to that of the wild type enzyme | Geobacillus stearothermophilus |
Y46G | behavior in NAD+ binding is similar to that of the wild type enzyme | Geobacillus stearothermophilus |
Y46G/S48G | positive cooperativity in binding the coenzyme NAD+ | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NAD+ | mutant enzyme S48G | Geobacillus stearothermophilus | |
0.05 | - |
NAD+ | wild-type enzyme | Geobacillus stearothermophilus | |
0.07 | - |
NAD+ | mutant enzyme E276G | Geobacillus stearothermophilus | |
0.33 | - |
NAD+ | mutant enzyme S186G | Geobacillus stearothermophilus | |
0.8 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme Y46G | Geobacillus stearothermophilus | |
0.9 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme E276G | Geobacillus stearothermophilus | |
1 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme D186G and mutant enzyme S48G | Geobacillus stearothermophilus | |
1.1 | - |
D-glyceraldehyde 3-phosphate | wild-type enzyme | Geobacillus stearothermophilus | |
7 | - |
phosphate | mutant enzyme E276G and mutant enzyme S48G | Geobacillus stearothermophilus | |
8 | - |
phosphate | mutant enzyme Y46G | Geobacillus stearothermophilus | |
18 | - |
phosphate | mutant enzyme D186G | Geobacillus stearothermophilus | |
37 | - |
phosphate | wild-type enzyme | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P00362 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Geobacillus stearothermophilus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | dimers generated from the tetrameric enzyme are inactive but exhibit cooperativity in NAD+ binding | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dimers generated from the tetrameric enzyme are inactive but exhibit cooperativity in NAD+ binding | Geobacillus stearothermophilus |