Literature summary for 1.2.1.12 extracted from

Didierjean, C.; Corbier, C.; Fatih, M.; Favier, F.; Boschi-Muller, S.; Branlant, G.; Aubry, A.
Crystal structure of two ternary complexes of phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus with NAD and D-glyceraldehyde 3-phosphate (2003), J. Biol. Chem., 278, 12968-12976.

Search for more literature for 1.2.1.12

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor-diffusion method, enzyme in complex with NAD+ and D-glyceraldehyde 3-phosphate. C149A GAPDH and C149S GAPDH ternary complexes are obtained by soaking the crystals of the corresponding binary complexes (enzyme -NAD+) in a solution containing D-glyceraldehyde 3-phosphate	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
C149A	mutant enzyme displays no significant dehydrogenase activity	Geobacillus stearothermophilus
C149S	low but significant phosphorylating dehydrogenase activity	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	P00362	-	-

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)