Any feedback?
Literature summary for 1.2.1.12 extracted from
- Reconstitution and properties of the recombinant glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase supramolecular complex of Arabidopsis (2005), Plant Physiol., 139, 1433-1443.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET28a vector for expression in Escherichia coli BL21DE3 cells, amplificatied in Escherichia coli HB101 cells | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | DTT | Arabidopsis thaliana |  |
| H2O2 | - |
Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
monomer, determined by SDS-PAGE | Arabidopsis thaliana |
| 120000 | - |
tetramer, determined by gel filtration | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glyceroyl phosphate + NADH + H+ | Arabidopsis thaliana | - |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification protocol comprises two anion-exchange chromatographic steps and removing of the His tag | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
Arabidopsis thaliana | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GapA | A4 isoform of GAPDH | Arabidopsis thaliana |
| GAPDH | - |
Arabidopsis thaliana |
| glyceraldehyde-3-phosphate dehydrogenase | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Arabidopsis thaliana | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
