Literature summary for 1.2.1.12 extracted from

Satchell, J.F.; Malby, R.L.; Luo, C.S.; Adisa, A.; Alpyurek, A.E.; Klonis, N.; Smith, B.J.; Tilley, L.; Colman, P.M.
Structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum (2005), Acta Crystallogr. Sect. D, D61, 1213-1221.

Search for more literature for 1.2.1.12

Application

Application	Comment	Organism
medicine	potential antimalarial target	Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment)	Organism
into the pET15b vector for expression in Escherichia coli BL21DE3 cells	Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of tag-free protein diffracts X-rays to 2.6 A resolution, of the His-tagged protein to 4.0 A	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
ferriprotoporphyrin IX	-	Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	monomer	Plasmodium falciparum
130000	-	tetramer, estimated by gel filtration	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	Plasmodium falciparum	-	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
the filtered supernatant of the centrifuged cell lysate is applied onto 3 ml Talon resin, the His-tag is removed, further purification by gel filtration	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Plasmodium falciparum	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
GAPDH	-	Plasmodium falciparum
glyceraldehyde-3-phosphate dehydrogenase	-	Plasmodium falciparum
PfGAPDH	-	Plasmodium falciparum

Cofactor

Cofactor	Comment	Organism	Structure
NADH	one molecule NAD+ is bound to each of the four subunits of the tetrameric enzyme	Plasmodium falciparum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)