Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Cryptosporidium parvum |
Crystallization (Comment) | Organism |
---|---|
GAPDH in the apo and holo (enzyme in complex with NAD+) state, hanging drop vapor diffusion method, using 16-20% (w/v) PEG 3000 and 0.2 M NaCl in 0.1 M HEPES, pH 7.5 | Cryptosporidium parvum |
Protein Variants | Comment | Organism |
---|---|---|
C153S | the specific activity of the mutant enzyme is approximately 450fold lower than that of the wild type enzyme | Cryptosporidium parvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000032 | - |
NAD+ | wild type enzyme, in 50 mM triethanolamine, 50 mM Na2HPO4, 0.2 mM EDTA, pH 8.8, temperature not specified in the publication | Cryptosporidium parvum | |
0.763 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 50 mM triethanolamine, 50 mM Na2HPO4, 0.2 mM EDTA, pH 8.8, temperature not specified in the publication | Cryptosporidium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | Q7YYQ9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, SP Sepharose column chromatography, and Superdex 200 gel filtration | Cryptosporidium parvum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Cryptosporidium parvum | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Cryptosporidium parvum |
Synonyms | Comment | Organism |
---|---|---|
GAPDH | - |
Cryptosporidium parvum |
glyceraldehyde 3-phosphate dehydrogenase | - |
Cryptosporidium parvum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cryptosporidium parvum |