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Literature summary for 1.2.1.12 extracted from
- Surface localized and extracellular glyceraldehyde-3-phosphate dehydrogenase of Bacillus anthracis is a plasminogen binding protein (2010), Biochim. Biophys. Acta, 1804, 2111-2120.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus anthracis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.366 | - |
NAD+ | pH 8.6, temperature not specified in the publication | Bacillus anthracis |  |
| 0.366 | - |
NAD+ | at pH 8.5, temperature not specified in the publication | Bacillus anthracis | |
| 1.78 | - |
3-phospho-D-glyceroyl phosphate | pH 8.6, temperature not specified in the publication | Bacillus anthracis | |
| 1.78 | - |
D-glyceraldehyde 3-phosphate | at pH 8.5, temperature not specified in the publication | Bacillus anthracis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Bacillus anthracis | 9986 | - |
| extracellular | - |
Bacillus anthracis | - |
- |
| membrane | - |
Bacillus anthracis | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Bacillus anthracis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Bacillus anthracis | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus anthracis | Q81X74 | - |
- |
| Bacillus anthracis | YP_027084 | isoform GapA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA affinity column chromatography | Bacillus anthracis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glyceroyl phosphate + NADH | - |
Bacillus anthracis | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? | |
| D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Bacillus anthracis | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Bacillus anthracis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GapA | - |
Bacillus anthracis |
| GapA | isoform | Bacillus anthracis |
| GAPDH | - |
Bacillus anthracis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 65 | - |
Bacillus anthracis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, no loss of activity | Bacillus anthracis |
| 40 | - |
the enzymatic activity is retained after incubation at 40°C for 30 min | Bacillus anthracis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bacillus anthracis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus anthracis | |
| NAD+ | isoform GapA is strictly dependent on NAD+ | Bacillus anthracis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | human plasminogen predominantly interacts with the GapA isoform at physiological concentrations and the interaction is lysine dependent | Bacillus anthracis |
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