Literature summary for 1.2.1.12 extracted from

Zhang, C.; Zhuge, B.; Zhan, X.; Fang, H.; Zong, H.; Zhuge, J.
Cloning and characterization of a novel NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase gene from Candida glycerinogenes and use of its promoter (2013), Yeast, 30, 157-163.

Cloned(Commentary)

Cloned (Comment)	Organism
gene CgGAP or GPD, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, promoter analysis, six stress-response elements were found in the upstream region of the CgGAP gene. Recombinant expression in enzyme-deficient Saccharomyces cerevisiae from plasmid pYX212-CgGAP and functional complementation restoring the wild-type phenotype. The GAP promoter (PCgGAP) is constitutively expressed in Saccharomyces cerevisiae cerevisiae cells grown on glucose, determined via GFP-tagged gene expression analysis	Pichia kudriavzevii

Cloned (Comment)

Organism

gene CgGAP or GPD, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, promoter analysis, six stress-response elements were found in the upstream region of the CgGAP gene. Recombinant expression in enzyme-deficient Saccharomyces cerevisiae from plasmid pYX212-CgGAP and functional complementation restoring the wild-type phenotype. The GAP promoter (PCgGAP) is constitutively expressed in Saccharomyces cerevisiae cerevisiae cells grown on glucose, determined via GFP-tagged gene expression analysis

Pichia kudriavzevii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	-	Pichia kudriavzevii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	Pichia kudriavzevii	-	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	Pichia kudriavzevii WL2002-5	-	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Pichia kudriavzevii	-	-	-
Pichia kudriavzevii WL2002-5	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Pichia kudriavzevii	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Pichia kudriavzevii WL2002-5	3-phospho-D-glyceroyl phosphate + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 36000, about, sequence determination and analysis	Pichia kudriavzevii
More	the CgGAP protein consists of an N-terminal NAD+-binding domain and a central catalytic domain	Pichia kudriavzevii

Synonyms

Synonyms	Comment	Organism
CgGAP	-	Pichia kudriavzevii
GAPDH	-	Pichia kudriavzevii
NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase	-	Pichia kudriavzevii

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pichia kudriavzevii

Expression

Organism	Comment	Expression
Pichia kudriavzevii	six stress-response elements were found in the upstream region of the CgGAP gene	additional information

General Information

General Information	Comment	Organism
metabolism	glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme of glycolysis, catalyses the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate using NAD+ as the co-enzyme	Pichia kudriavzevii