Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | required | Haemonchus contortus |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli | Haemonchus contortus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is part of the excretory-secretory products of the parasite | Haemonchus contortus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Haemonchus contortus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | 150000 | native PAGE | Haemonchus contortus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Haemonchus contortus | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemonchus contortus | H6WVX3 | propagated in sheep and goat, isolated from sheep and goat abomasums (stomachs) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Haemonchus contortus | - |
larva | - |
Haemonchus contortus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
freshly isolated enzyme, complement-C3-binding protein activity, pH 8.0, temperature not specified in the publication | Haemonchus contortus |
1 | - |
isolated enzyme from fresh parasites, GAPDH activity, pH 8.0, temperature not specified in the publication | Haemonchus contortus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Haemonchus contortus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 14000, SDS-PAGE, probably truncated form, x * 37000, native enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Haemonchus contortus |
More | isolation of a truncated form of the protein from the excretory-secretory products of the parasite worms using C3-affinity chromatography, and identification by mass spectroscopy as glyceraldehyde-3-phosphate dehydrogenase, GAPDH | Haemonchus contortus |
Synonyms | Comment | Organism |
---|---|---|
complement-C3-binding protein | - |
Haemonchus contortus |
GAPDH | - |
Haemonchus contortus |
glyceraldehyde-3-phosphate dehydrogenase | - |
Haemonchus contortus |
H.c-C3BP | - |
Haemonchus contortus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Haemonchus contortus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Haemonchus contortus | |
NADH | - |
Haemonchus contortus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme inhibits complement function as measured by haemolytic assay and membrane attack complex (MAC) formation, C3-binding property of Haemonchus contortus GAPDH is an additional function of the enzyme, and it represents another entity of complement-binding protein. Key role of the protein in immune modulation | Haemonchus contortus |