Protein Variants | Comment | Organism |
---|---|---|
C150G | the Tdh3 mutation eliminates catalytic activity, the mutant is defective in silencing and deficient in NAD+ binding | Saccharomyces cerevisiae |
additional information | nuclear export sequences are fused to the 3' end of gene TDH3 by transforming a DNA fragment with 3' homology to the TDH3 ORF, the nuclear export sequence, and an hphMX4 sequence into the appropriate yeast strain. Strains lacking both TDH3 and specific NAD+ biosynthetic genes are generated by crossing Dtdh3 strain YSH969 with selected strains from the yeast deletion collection. Identification of sporulation haploid strains | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Saccharomyces cerevisiae | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Saccharomyces cerevisiae YSH503 | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae YSH503 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Saccharomyces cerevisiae | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Saccharomyces cerevisiae YSH503 | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glyceraldehyde 3-phosphate dehydrogenase | - |
Saccharomyces cerevisiae |
TDH3 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | TDH3 deletion or overexpression does not affect overall cellular NAD+ levels, but affects nuclear NAD+ levels in yeast | Saccharomyces cerevisiae |
physiological function | Tdh3, an NAD+-binding protein, influences nuclear NAD+ levels. Tdh3 links nuclear Sir2 with NAD+ from the cytoplasm | Saccharomyces cerevisiae |