Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.12 extracted from

  • Wang, S.; Chen, H.; Tang, X.; Zhang, H.; Hao, G.; Chen, W.; Chen, Y.Q.
    The role of glyceraldehyde-3-phosphate dehydrogenases in NADPH supply in the oleaginous filamentous fungus Mortierella alpina (2020), Front. Microbiol., 11, 818 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
gene GAPDH1, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, recombinant overexpression in Mortierella alpina using Agrobacterium tumefaciens-mediated transformation Mortierella alpina
gene GAPDH2, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, recombinant expression using Agrobacterium tumefaciens-mediated transformation Mortierella alpina

Protein Variants

Protein Variants Comment Organism
additional information GAPDH1 gene silencing using Agrobacterium tumefaciens strain AGL-1-mediated transformation o the cells with siRNA. RNA interference of isozymes GAPDH1 and GAPDH2 (MA-RGAPDH1 and MA-RGAPDH2) greatly reduced the biomass of the fungus. The lipid content of MA-RGAPDH2 is about 23% higher than that of the control. Both of the lipid-increasing transformants show a higher NADPH/NADP ratio. Analysis of metabolite and enzyme expression levels reveals that the increased lipid content of MA-GAPDH1 is due to enhanced flux of glyceraldehyde-3-phosphate to glycerate-1,3-biphosphate. MA-RGAPDH2 is found to strengthen the metabolic flux of dihydroxyacetone phosphate to glycerol-3-phosphate Mortierella alpina
additional information GAPDH1 gene silencing using Agrobacterium tumefaciens strain AGL-1-mediated transformation of the cells with siRNA. RNA interference of isozymes GAPDH1 and GAPDH2 (MA-RGAPDH1 and MA-RGAPDH2) greatly reduced the biomass of the fungus. The lipid content of MA-RGAPDH2 is about 23% higher than that of the control. Both of the lipid-increasing transformants show a higher NADPH/NADP ratio. Analysis of metabolite and enzyme expression levels reveals that the increased lipid content of MA-GAPDH1 is due to enhanced flux of glyceraldehyde-3-phosphate to glycerate-1,3-biphosphate Mortierella alpina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Mortierella alpina
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Mortierella alpina ATCC 32222
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Mortierella alpina A0A650AW89
-
-
Mortierella alpina A0A650AW98
-
-
Mortierella alpina ATCC 32222 A0A650AW89
-
-
Mortierella alpina ATCC 32222 A0A650AW98
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Mortierella alpina 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Mortierella alpina ATCC 32222 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GAPDH
-
 Mortierella alpina
GAPDH1
-
 Mortierella alpina
GAPDH2
-
 Mortierella alpina
glyceraldehyde-3-phosphate dehydrogenases
-
 Mortierella alpina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mortierella alpina

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Mortierella alpina

General Information

General Information Comment Organism
malfunction RNA interference of isozymes GAPDH1 and GAPDH2 (MA-RGAPDH1 and MA-RGAPDH2) greatly reduced the biomass of the fungus. The lipid content of MA-RGAPDH2 is about 23% higher than that of the control. Both of the lipid-increasing transformants show a higher NADPH/NADP ratio. Analysis of metabolite and enzyme expression levels reveals that the increased lipid content of MA-GAPDH1 is due to enhanced flux of glyceraldehyde-3-phosphate to glycerate-1,3-biphosphate. Genetic manipulation of gapdh1 and gapdh2 affects the biomass and total fatty acid of Mortierella alpina through an altered NADPH/NADP ratio Mortierella alpina
malfunction RNA interference of isozymes GAPDH1 and GAPDH2 (MA-RGAPDH1 and MA-RGAPDH2) greatly reduced the biomass of the fungus. The lipid content of MA-RGAPDH2 is about 23% higher than that of the control. Both of the lipid-increasing transformants show a higher NADPH/NADP ratio. Analysis of metabolite and enzyme expression levels reveals that the increased lipid content of MA-GAPDH1 is due to enhanced flux of glyceraldehyde-3-phosphate to glycerate-1,3-biphosphate. MA-RGAPDH2 is found to strengthen the metabolic flux of dihydroxyacetone phosphate to glycerol-3-phosphate. Genetic manipulation of gapdh1 and gapdh2 affects the biomass and total fatty acid of Mortierella alpina through an altered NADPH/NADP ratio Mortierella alpina
metabolism different roles of GAPDH1 and GAPDH2 in lipid biosynthesis in Mortierella alpina Mortierella alpina
metabolism different roles of GAPDH1 and GAPDH2 in lipid biosynthesis in Mortierella alpina. The GAPDH2 might be a moonlighting protein Mortierella alpina
physiological function isozyme GAPDH1 contributes to NADPH supply and lipid accumulation in Mortierella alpina, and has a distinct role from isozyme GAPDH2. Transcriptional analysis of genes gapdh1 and gapdh2 shows that they have opposing roles during lipid accumulation. GAPDH1 possesses a stronger catalyzing ability than GAPDH2 Mortierella alpina