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Literature summary for 1.2.1.13 extracted from

  • Russo, A.D.; Rullo, R.; Masullo, M.; Ianniciello, G.; Arcari, P.; Bocchini, V.
    Glyceraldehyde 3-phosphate dehydrogenase in the hyperthermophilic archaeon Sulfolobus solfataricus: characterization and significance in glucose metabolism (1995), Biochem. Mol. Biol. Int., 36, 123-135.
    View publication on PubMed
Search for more literature for 1.2.1.13

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADPH
-
 Saccharolobus solfataricus
0.02
-
 NADP+
-
 Saccharolobus solfataricus
0.1
-
 NADP+
-
 Saccharolobus solfataricus
0.18
-
 NADH
-
 Saccharolobus solfataricus
5
-
 NAD+
-
 Saccharolobus solfataricus
88
-
 arsenate
-
 Saccharolobus solfataricus
182
-
 phosphate
-
 Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 4 * 41000, SDS-PAGE Saccharolobus solfataricus
155000
-
 gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.2
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + arsenate + NADP+
-
 Saccharolobus solfataricus 3-phospho-D-glyceroyl arsenate + NADPH
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Saccharolobus solfataricus 3-phospho-D-glyceroyl phosphate + NADH
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Saccharolobus solfataricus 3-phospho-D-glyceroyl phosphate + NADPH
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
87
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 40 min, stable Saccharolobus solfataricus
87
-
 half-life: 45 min Saccharolobus solfataricus
95
-
 30 min, complete inactivation Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 both directions Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+ activity with NADP+ is about 50fold higher than that determined for NAD+ Saccharolobus solfataricus
NADP+ cofactor Saccharolobus solfataricus