Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Spinacia oleracea |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with 2.0-2.5 M or 1.5-1.2 M ammonium sulfate and 0.1 M potassium phosphate (pH 7.0-8.0) | Spinacia oleracea |
Protein Variants | Comment | Organism |
---|---|---|
D351N | the mutation only marginally affects the redox sensitivity | Spinacia oleracea |
E356Q | the mutation only marginally affects the redox sensitivity | Spinacia oleracea |
E356Q/E357Q | complete redox insensitivity is achieved in the double mutant | Spinacia oleracea |
E357Q | the mutation only marginally affects the redox sensitivity | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | P19866 | - |
- |
Oxidation Stability | Organism |
---|---|
oxidation typically leads to about 50% inhibition of the NADPH-dependent activity | Spinacia oleracea |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | fully active enzyme | Spinacia oleracea |
Synonyms | Comment | Organism |
---|---|---|
A2B2-GAPDH | fully active enzyme | Spinacia oleracea |
A2B2-glyceraldehyde-3-phosphate dehydrogenase | - |
Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | does also accept NAD+ but to a lower degree than NADP+ | Spinacia oleracea | |
NADP+ | preferred cofactor | Spinacia oleracea |