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Literature summary for 1.2.1.16 extracted from

  • Alhasawi, A.A.; Thomas, S.C.; Tharmalingam, S.; Legendre, F.; Appanna, V.D.
    Isocitrate lyase and succinate semialdehyde dehydrogenase mediate the synthesis of alpha-ketoglutarate in Pseudomonas fluorescens (2019), Front. Microbiol., 10, 1929 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene SSADH, quantitative real-time PCR enzyme expression analysis Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
sodium arsenite inhibits the activity of SSADH Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinate semialdehyde + NAD+ + H2O Pseudomonas fluorescens
-
 succinate + NADH + 2 H+
-
 r
succinate semialdehyde + NAD+ + H2O Pseudomonas fluorescens ATCC 13525
-
 succinate + NADH + 2 H+
-
 r
succinate semialdehyde + NADP+ + H2O Pseudomonas fluorescens
-
 succinate + NADPH + 2 H+
-
 r
succinate semialdehyde + NADP+ + H2O Pseudomonas fluorescens ATCC 13525
-
 succinate + NADPH + 2 H+
-
 r

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens A0A0F4THK8
-
-
Pseudomonas fluorescens ATCC 13525 A0A0F4THK8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NAD+ + H2O
-
 Pseudomonas fluorescens succinate + NADH + 2 H+
-
 r
succinate semialdehyde + NAD+ + H2O
-
 Pseudomonas fluorescens ATCC 13525 succinate + NADH + 2 H+
-
 r
succinate semialdehyde + NADP+ + H2O
-
 Pseudomonas fluorescens succinate + NADPH + 2 H+
-
 r
succinate semialdehyde + NADP+ + H2O
-
 Pseudomonas fluorescens ATCC 13525 succinate + NADPH + 2 H+
-
 r

Synonyms

Synonyms Comment Organism
SSADH
-
 Pseudomonas fluorescens
succinic semialdehyde dehydrogenase
-
 Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas fluorescens
NADH
-
 Pseudomonas fluorescens
NADP+
-
 Pseudomonas fluorescens
NADPH
-
 Pseudomonas fluorescens

General Information

General Information Comment Organism
metabolism the enhanced production of 2-oxoglutarate (KG) effected by Pseudomonas fluorescens in a mineral medium supplemented with manganese (Mn) is propelled by the increased activities of succinate semialdehyde dehydrogenase (SSADH), gamma-aminobutyric acid aminotransaminase (GABAT), and isocitrate lyase (ICL). The latter generates glyoxylate and succinate two key metabolites involved in this process. Fumarate reductase (FRD) also aids in augmenting the pool of succinate, a precursor of succinate semialdehyde (SSA). The latter is then carboxylated to KG with the assistance of 2-oxoglutarate decarboxylase (KDC) Pseudomonas fluorescens