Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tris buffer | - |
Cupriavidus oxalaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + CoA + NADP+ | Pseudomonas sp. | - |
oxalyl-CoA + NADPH | - |
r | |
glyoxylate + CoA + NADP+ | Cupriavidus oxalaticus | - |
oxalyl-CoA + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus oxalaticus | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using protamine sulfate treatment, ammonium sulfate precipitation and dialysis, alumina C-gamma adsorption, concentration of the enzyme by ammonium sulfate precipitation followed by dialysis and ion exchange chromatography on DEAE-cellulose | Cupriavidus oxalaticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.2 | - |
- |
Cupriavidus oxalaticus |
7.2 | - |
- |
Cupriavidus oxalaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + CoA + NADP+ | - |
Pseudomonas sp. | oxalyl-CoA + NADPH | - |
r | |
glyoxylate + CoA + NADP+ | - |
Cupriavidus oxalaticus | oxalyl-CoA + NADPH | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
glyoxylate + CoA + NADP+ | Cupriavidus oxalaticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Pseudomonas sp. | |
CoA | specific for | Cupriavidus oxalaticus | |
NADP+ | - |
Pseudomonas sp. | |
NADP+ | specific for | Cupriavidus oxalaticus |