Literature summary for 1.2.1.18 extracted from

Hayaishi, O.; Nishizuka, Y.; Tatibana, M.; Takeshita, M.; Kuno, S.
Enzymatic studies on the metabolism of beta-alanine (1961), J. Biol. Chem., 236, 781-790.

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Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	0.1 M required for maximal activity	Pseudomonas fluorescens

General Stability

General Stability	Organism
dialysis against phosphate buffer, pH 7.0, for 4 h at 4°C results in 80% loss of activity	Pseudomonas fluorescens
freezing and thawing causes total loss of activity even in the presence of 0.01 M 2-mercaptoethanol	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Pseudomonas fluorescens

Storage Stability

Storage Stability	Organism
2°C, pH 7.0, in presence of 2-mercaptoethanol, stored with least loss of activity	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-oxopropanoate + CoA + NAD+	-	Pseudomonas fluorescens	acetyl-CoA + CO2 + NADH	-	ir

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	6	activity with NADP+	Pseudomonas fluorescens
8.8	-	activity with NAD+	Pseudomonas fluorescens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	cofactor	Pseudomonas fluorescens
NADP+	cofactor	Pseudomonas fluorescens

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Release 2023.1 (January 2023)