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Literature summary for 1.2.1.19 extracted from

  • Yin, Y.; Yang, R.; Wu, J.; Gu, Z.
    Partial purification, characterization and cDNA cloning of aminoaldehyde dehydrogenase in germinated soybean (Glycine max L.) (2013), Eur. Food Res. Technol., 237, 731-738 .
No PubMed abstract available
Search for more literature for 1.2.1.19

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons Glycine max

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 66% inhibition at 1 mM Glycine max
Ba2+ 30% inhibition at 1 mM Glycine max
Cu2+ complete inhibition at 1 mM Glycine max
EGTA 7.5% inhibition at 1 mM Glycine max
Hg2+ 62% inhibition at 1 mM Glycine max
L-cysteine 59% inhibition at 1 mM Glycine max
Mg2+ 70% inhibition at 1 mM Glycine max
additional information EDTA has no significant effect on the enzyme activity Glycine max
Ni2+ complete inhibition at 1 mM Glycine max
Pb2+ complete inhibition at 1 mM Glycine max
Zn2+ 92% inhibition at 1 mM Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no significant effect on the enzyme activity Glycine max
Mn2+ no significant effect on the enzyme activity Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max U5ILS4
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 27.22fold from germinated seeds by ammonium sulfate fractionation, desalting gel filtration, and anion exchange chromatography Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seed germinated Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.71
-
 purified native enzyme, substrate 4-aminobutanal, pH 8.0, 37°C Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-aminopropionaldehyde + NAD+ + H2O best substrate Glycine max 3-aminopropionate + NADH + 2 H+
-
 ?
4-aminobutyraldehyde + NAD+ + H2O
-
 Glycine max 4-aminobutyrate + NADH + 2 H+
-
 ?
additional information no activity with betaine aldehyde, propionaldehyde, and acetaldehyde Glycine max ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53720, native enzyme, SDS-PAGE, x * 54790, sequence calculation Glycine max

Synonyms

Synonyms Comment Organism
AMADH
-
 Glycine max
aminoaldehyde dehydrogenase
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Glycine max

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 activity range, profile overview Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 purified enzyme, pH 8.0, rapid inactivation above 50°C, loss of almost 50% activity at 60°C Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
3 9 AMADH activity increases with a pH ranging from pH 3.0 to pH 8.0, but it decreases drastically when pH continuously rises from pH 8.0 to pH 10.0. At pH 9.0, the activity decreases by 75% compared to pH 8.0 Glycine max

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Glycine max

pI Value

Organism Comment pI Value Maximum pI Value
Glycine max sequence calculation
-
 5.16

General Information

General Information Comment Organism
evolution the enzyme is a member of the aldehyde dehydrogenase 10 family Glycine max