Cloned (Comment) | Organism |
---|---|
gene gadbh, quantitative RT-PCR enzyme expression analysis | Synechocystis sp. PCC 6803 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a series of Synechocystis sp. PCC6803 mutants lacking enzymes of TCAC shunt, GABA synthesis and polyamine degradation pathway, i.e. DELTAkgd, DELTAgad:DELTAkgd and DELTAgabdh encoding glutamate and 2-oxoglutarate decarboxylases, respectively. Subcloning in Escherichia coli strain DH5alpha. The level of chl a content is significantly decreased in both DELTAgabdh and DELTAgad:DELTAkgd strains as compared to that in the wild-type. Similarly, lower O2 evolution rate is observed in both mutants signifying a decline in photosynthetic efficiency, phenotype, overview. The mutant strain with disrupted gene gabdh shows an increase in gamma-aminobutyric acid (GABA), glutamate, succinate, and spermidine levels, while the DELTAgad:DELTAkgd strain shows upregulated expression of gene gadbh | Synechocystis sp. PCC 6803 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutyraldehyde + NAD+ + H2O | Synechocystis sp. PCC 6803 | - |
4-aminobutanoate + NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. PCC 6803 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutyraldehyde + NAD+ + H2O | - |
Synechocystis sp. PCC 6803 | 4-aminobutanoate + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gadbh | - |
Synechocystis sp. PCC 6803 |
gamma-aminobutanal dehydrogenase | - |
Synechocystis sp. PCC 6803 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Synechocystis sp. PCC 6803 |
General Information | Comment | Organism |
---|---|---|
malfunction | a mutant strain with disrupted gene gabdh shows an increase in gamma-aminobutyric acid (GABA), glutamate, succinate, and spermidine levels demonstrating a link between spermidine degradation and GABA synthesis in cyanobacteria. A DELTAgad:DELTAkgd strain shows upregulated expression of gene gadbh. Transcription levels of genes related to GABA metabolism in WT and mutants, overview | Synechocystis sp. PCC 6803 |
physiological function | role of gamma-aminobutanal dehydrogenase in maintaining an intact tricarboxylic acid cycle in Synechocystis. The mutational analysis suggests a possible existence of the Spd to gamma-AB to GABA pathway in Synechocystis | Synechocystis sp. PCC 6803 |