BRENDA - Enzyme Database
show all sequences of 1.2.1.20

Prediction of missing enzyme genes in a bacterial metabolic network. Reconstruction of the lysine-degradation pathway of Pseudomonas aeruginosa

Yamanishi, Y.; Mihara, H.; Osaki, M.; Muramatsu, H.; Esaki, N.; Sato, T.; Hizukuri, Y.; Goto, S.; Kanehisa, M.; FEBS J. 274, 2262-2273 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
into pET21a(+) vector, proteins with a C-terminal His6-tag expressed in Escherichia coli BL21(DE3)
Pseudomonas aeruginosa PAO1
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Pseudomonas aeruginosa PAO1
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa PAO1
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
to homogeneity as a C-terminal histidine-tagged fusion protein
Pseudomonas aeruginosa PAO1
Storage Stability
Storage Stability
Organism
-80°C, 20 mm Tris-HCl buffer, pH 8.0
Pseudomonas aeruginosa PAO1
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
glutarate semialdehyde + NADP+ + H2O
-
673588
Pseudomonas aeruginosa PAO1
glutarate + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 53000, SDS-PAGE
Pseudomonas aeruginosa PAO1
Synonyms
Synonyms
Commentary
Organism
glutarate semialdehyde dehydrogenase
-
Pseudomonas aeruginosa PAO1
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas aeruginosa PAO1
Cloned(Commentary) (protein specific)
Commentary
Organism
into pET21a(+) vector, proteins with a C-terminal His6-tag expressed in Escherichia coli BL21(DE3)
Pseudomonas aeruginosa PAO1
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Pseudomonas aeruginosa PAO1
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Pseudomonas aeruginosa PAO1
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity as a C-terminal histidine-tagged fusion protein
Pseudomonas aeruginosa PAO1
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, 20 mm Tris-HCl buffer, pH 8.0
Pseudomonas aeruginosa PAO1
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
glutarate semialdehyde + NADP+ + H2O
-
673588
Pseudomonas aeruginosa PAO1
glutarate + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 53000, SDS-PAGE
Pseudomonas aeruginosa PAO1
Other publictions for EC 1.2.1.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721885
Adkins
Engineering Escherichia coli f ...
Pseudomonas putida, Pseudomonas putida KT 2240
Biotechnol. Bioeng.
110
1726-1734
2013
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-
1
-
-
-
-
-
-
-
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2
-
42
-
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-
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2
-
2
-
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1
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2
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2
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-
743218
Park
Metabolic engineering of Esch ...
Pseudomonas putida, Pseudomonas putida ATCC 47054
Metab. Eng.
16
42-47
2013
-
-
1
-
1
-
-
-
-
-
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2
-
6
-
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2
-
2
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1
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1
1
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1
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2
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-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
673588
Yamanishi
Prediction of missing enzyme g ...
Pseudomonas aeruginosa PAO1
FEBS J.
274
2262-2273
2007
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-
1
-
-
-
-
-
-
-
1
-
-
7
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1
-
-
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1
1
1
1
-
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1
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1
1
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1
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1
-
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1
1
1
-
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-
288128
Rhee
Anaerobic and aerobic degradat ...
Bacteria
Appl. Environ. Microbiol.
63
2578-2585
1997
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
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-
-
-
-
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3
-
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-
-
-
-
-
-
2
-
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2
-
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1
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3
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-
288124
Chang
Factors influencing growth on ...
Pseudomonas putida
J. Biol. Chem.
252
7987-7991
1977
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2
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1
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3
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1
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1
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2
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3
-
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-
288126
Chang
Glutarate semialdehyde dehydro ...
Pseudomonas putida
J. Biol. Chem.
252
7979-7986
1977
-
-
-
-
-
-
2
-
-
-
-
1
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5
-
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1
-
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1
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3
-
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1
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1
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2
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1
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1
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1
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3
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-
288125
Chang
-
Factors influencing growth on ...
Pseudomonas putida
Methods Enzymol.
17B
166-171
1971
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-
-
-
-
-
-
-
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-
1
1
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1
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1
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1
1
10
-
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1
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1
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1
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1
1
-
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1
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1
1
10
-
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-
-
-
1
-
-
-
-
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-
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288127
Ichihara
Metabolism of L-lysine by bact ...
Pseudomonas sp.
J. Biochem.
49
154-157
1961
-
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-
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2
2
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1
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1
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2
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1
1
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2
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2
-
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2
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2
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1
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2
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1
1
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