BRENDA - Enzyme Database
show all sequences of 1.2.1.20

Metabolic engineering of Escherichia coli for the production of 5-aminovalerate and glutarate as C5 platform chemicals

Park, S.; Kim, E.; Noh, W.; Park, H.; Oh, Y.; Lee, S.; Song, B.; Jegal, J.; Lee, S.; Metab. Eng. 16, 42-47 (2013)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene davD, recombinant expression in Escherichia coli strain W3110, coexpression with Pseudomonas putida gene gabT, encoding 5-aminovalerate aminotransferase, and davA and davB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively. The recombinant Escherichia coli WL3110 strain expressingthe davAB and gabTD genes is cultured in a medium containing D-glucose, L-lysine, and 2-oxoglutarate, and produces glutarate
Pseudomonas putida
Engineering
Protein Variants
Commentary
Organism
additional information
metabolic engineering strategies for the production of 5-aminovalerate from L-lysine in vivo synthesized from glucose in Escherichia coli by amplifying metabolic fluxes and activities, e.g. enzyme glutarate semialdehyde dehydrogenase, and by removing repressions and feedback inhibitions involved in L-lysine metabolism, oevrview
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glutarate semialdehyde + NAD+ + H2O
Pseudomonas putida
-
glutarate + NADH + 2 H+
-
-
?
glutarate semialdehyde + NAD+ + H2O
Pseudomonas putida ATCC 47054
-
glutarate + NADH + 2 H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
Q88RC0
-
-
Pseudomonas putida ATCC 47054
Q88RC0
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
glutarate semialdehyde + NAD+ + H2O
-
743218
Pseudomonas putida
glutarate + NADH + 2 H+
-
-
-
?
glutarate semialdehyde + NAD+ + H2O
-
743218
Pseudomonas putida ATCC 47054
glutarate + NADH + 2 H+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
davD
-
Pseudomonas putida
glutarate semialdehyde dehydrogenase
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
gene davD, recombinant expression in Escherichia coli strain W3110, coexpression with Pseudomonas putida gene gabT, encoding 5-aminovalerate aminotransferase, and davA and davB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively. The recombinant Escherichia coli WL3110 strain expressingthe davAB and gabTD genes is cultured in a medium containing D-glucose, L-lysine, and 2-oxoglutarate, and produces glutarate
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
metabolic engineering strategies for the production of 5-aminovalerate from L-lysine in vivo synthesized from glucose in Escherichia coli by amplifying metabolic fluxes and activities, e.g. enzyme glutarate semialdehyde dehydrogenase, and by removing repressions and feedback inhibitions involved in L-lysine metabolism, oevrview
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glutarate semialdehyde + NAD+ + H2O
Pseudomonas putida
-
glutarate + NADH + 2 H+
-
-
?
glutarate semialdehyde + NAD+ + H2O
Pseudomonas putida ATCC 47054
-
glutarate + NADH + 2 H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
glutarate semialdehyde + NAD+ + H2O
-
743218
Pseudomonas putida
glutarate + NADH + 2 H+
-
-
-
?
glutarate semialdehyde + NAD+ + H2O
-
743218
Pseudomonas putida ATCC 47054
glutarate + NADH + 2 H+
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the metabolic pathways for the production of 5-aminovalerate and glutarate from L-lysine
Pseudomonas putida
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the metabolic pathways for the production of 5-aminovalerate and glutarate from L-lysine
Pseudomonas putida
Other publictions for EC 1.2.1.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721885
Adkins
Engineering Escherichia coli f ...
Pseudomonas putida, Pseudomonas putida KT 2240
Biotechnol. Bioeng.
110
1726-1734
2013
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42
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2
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743218
Park
Metabolic engineering of Esch ...
Pseudomonas putida, Pseudomonas putida ATCC 47054
Metab. Eng.
16
42-47
2013
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1
-
1
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6
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2
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2
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1
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1
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1
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2
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1
1
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673588
Yamanishi
Prediction of missing enzyme g ...
Pseudomonas aeruginosa PAO1
FEBS J.
274
2262-2273
2007
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1
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1
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7
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1
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1
1
1
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1
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1
1
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1
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1
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1
1
1
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-
-
-
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-
288128
Rhee
Anaerobic and aerobic degradat ...
Bacteria
Appl. Environ. Microbiol.
63
2578-2585
1997
-
-
-
-
-
-
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-
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1
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1
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3
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2
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2
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1
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3
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288124
Chang
Factors influencing growth on ...
Pseudomonas putida
J. Biol. Chem.
252
7987-7991
1977
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2
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1
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3
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1
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1
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2
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3
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288126
Chang
Glutarate semialdehyde dehydro ...
Pseudomonas putida
J. Biol. Chem.
252
7979-7986
1977
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2
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1
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5
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1
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1
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3
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1
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3
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288125
Chang
-
Factors influencing growth on ...
Pseudomonas putida
Methods Enzymol.
17B
166-171
1971
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-
-
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1
1
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1
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1
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1
1
10
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1
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1
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1
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1
1
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1
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1
1
10
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1
-
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288127
Ichihara
Metabolism of L-lysine by bact ...
Pseudomonas sp.
J. Biochem.
49
154-157
1961
-
-
-
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2
2
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1
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1
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2
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1
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2
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