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Literature summary for 1.2.1.20 extracted from

  • Kim, H.T.; Khang, T.U.; Baritugo, K.A.; Hyun, S.M.; Kang, K.H.; Jung, S.H.; Song, B.K.; Park, K.; Oh, M.K.; Kim, G.B.; Kim, H.U.; Lee, S.Y.; Park, S.J.; Joo, J.C.
    Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical (2019), Metab. Eng., 51, 99-109 .
    View publication on PubMed
Search for more literature for 1.2.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene davD, recombinant expression in Corynebacterium glutamicum strain KCTC 1857, coexpression with 5-aminovalerate transaminase (davT, EC 2.6.1.48) from Pseudomonas putida, 4-aminobutyrate-2-oxoglutarate transaminase (gabT, EC 2.6.1.19) from Corynebacterium glutamicum, and NADP-dependent succinic semialdehyde dehydrogenase (gabD, EC 1.2.1.79) from Corynebacterium glutamicum, as well as N-terminal His6-tagged lysine 2-monooxygenase (davB, EC 1.13.12.2) from Pseudomonas putida Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical, by co-expression of Pseudomonas putida davT, davB, and davD genes encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively, in Corynebacterium glutamicum. The glutaric acid biosynthesis pathway constructed in recombinant Corynebacterium glutamicum is engineered by examining strong synthetic promoters H30 and H36, Corynebacterium glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His6-tag to improve the production of glutaric acid. The use of N-terminal His6-tagged DavB is most suitable for the production of glutaric acid from glucose. Fed-batch fermentation on of the final engineered Corynebacterium glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His6-tag at N-terminus can produce 24.5 g/l of glutaric acid with low accumulation of L-lysine (1.7 g/l), wherein 5-aminovaleric acid (5-AVA) ccumulation is not observed during fermentation. Metabolically engineered Corynebacterium glutamicum strain H30_GA-2 (engineered strain KCTC 1857) is able for catalysis of the biosynthesis of glutaric acid from glucose. Method optimization and evaluation, overview Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-oxopentanoate + NADP+ + H2O Pseudomonas putida
-
 glutarate + NADPH + H+
-
 ?
5-oxopentanoate + NADP+ + H2O Pseudomonas putida DSM 6125
-
 glutarate + NADPH + H+
-
 ?
5-oxopentanoate + NADP+ + H2O Pseudomonas putida NCIMB 11950
-
 glutarate + NADPH + H+
-
 ?
5-oxopentanoate + NADP+ + H2O Pseudomonas putida ATCC 47054
-
 glutarate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88RC0
-
-
Pseudomonas putida ATCC 47054 Q88RC0
-
-
Pseudomonas putida DSM 6125 Q88RC0
-
-
Pseudomonas putida NCIMB 11950 Q88RC0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-oxopentanoate + NADP+ + H2O
-
 Pseudomonas putida glutarate + NADPH + H+
-
 ?
5-oxopentanoate + NADP+ + H2O
-
 Pseudomonas putida DSM 6125 glutarate + NADPH + H+
-
 ?
5-oxopentanoate + NADP+ + H2O
-
 Pseudomonas putida NCIMB 11950 glutarate + NADPH + H+
-
 ?
5-oxopentanoate + NADP+ + H2O
-
 Pseudomonas putida ATCC 47054 glutarate + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
davD
-
 Pseudomonas putida
glutarate semialdehyde dehydrogenase
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Pseudomonas putida

General Information

General Information Comment Organism
metabolism glutarate semialdehyde dehydrogenase encoded by davD converts glutarate semialdehyde into glutaric acid. In the natural L-lysine catabolic pathway of Pseudomonas strains, glutaric acid is then further converted to acetyl-CoA, a main intermediate of Krebs cycle Pseudomonas putida