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Literature summary for 1.2.1.23 extracted from

  • Dunkerton, J.; James, S.P.
    Some observations on the NADP+-linked oxidation of methylglyoxal catalysed by 2-Oxoaldehyde dehydrogenase (1976), Biochem. J., 153, 503-504.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.23

Activating Compound

Activating Compound Comment Organism Structure
L-2-Aminopropan-1-ol activation of aldehyde oxidation Ovis aries
phosphate activation Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 NAD+
-
 Ovis aries
2.08
-
 methylglyoxal
-
 Ovis aries
2.08
-
 methylglyoxal in the presence of L-2-aminopropan-1-ol (Km 5.0 mM), at pH 9.3 and 25°C Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + NAD+ + H2O Ovis aries i.e. pyruvaldehyde pyruvate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation and column chromatography on DEAE-cellulose, phosphocellulose, hydroxyapatite and Sephadex G-200 Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoaldehydes + NAD+ + H2O enzyme from sheep or rat liver also active with NADP+, see also EC 1.2.1.49 Ovis aries 2-oxo acids + NADH
-
 ?
methylglyoxal + NAD+ + H2O i.e. pyruvaldehyde Ovis aries pyruvate + NADH + H+
-
 ?
additional information the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme Ovis aries ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Ovis aries
NADP+ enzyme from sheep or rat liver, see also EC 1.2.1.49 Ovis aries