Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | loss of about 50% of original acitivity after exhaustive dialysis, renaturation in presence of 2-mercaptoethanol | Escherichia coli K-12 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
NAD+ | pH 9.0, 37°C | Escherichia coli K-12 | |
0.047 | - |
succinate semialdehyde | pH 9.0, 37°C | Escherichia coli K-12 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
- |
- |
Storage Stability | Organism |
---|---|
-15°C, 14 h, 95% residual activity | Escherichia coli K-12 |
18°C, 14 h, 93% residual activity | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: formaldehyde, acetaldehyde, glyoxal, glyoxalate, propanal, glutaraldehyde, benzaldehyde, and anisaldehyde | Escherichia coli K-12 | ? | - |
? | |
n-butanal + NAD+ + H2O | about 11% of the rate with succinic semialdehyde | Escherichia coli K-12 | butanoic acid + NADH + 2 H+ | - |
? | |
succinate semialdehyde + NAD+ + H2O | - |
Escherichia coli K-12 | succinate + NADH + 2 H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli K-12 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
18 | - |
14 h, 93% residual activity | Escherichia coli K-12 |
37 | - |
14 h, 92% residual activity | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Escherichia coli K-12 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
rapid decrease in stability above | Escherichia coli K-12 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli K-12 |
Organism | Comment | Expression |
---|---|---|
Escherichia coli K-12 | induced by growth of cells on gamma-aminobutanoate | up |