Application | Comment | Organism |
---|---|---|
degradation | involved in an alternative pathway of D-glucarate metabolism | Acinetobacter baylyi |
degradation | involved in an alternative pathway of D-glucarate metabolism | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
expression in Escherichia coli BL21 (DE3) | Acinetobacter baylyi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | alpha-KGSA dehydrogenase activity in cell extracts from the mutant DELTAACIAD0131 grown on succinate as the sole carbon source is undetectable | Acinetobacter baylyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibitory effect with alpha-ketoglutarate or glutamate | Acinetobacter baylyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
alpha-Ketoglutaric semialdehyde | - |
Pseudomonas putida | |
0.015 | - |
NADP+ | - |
Acinetobacter baylyi | |
0.1 | - |
Glutaraldehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
0.3 | - |
propanal | with NADP+ as cofactor | Acinetobacter baylyi | |
0.31 | - |
Glutaraldehyde | with NAD+ as cofactor | Acinetobacter baylyi | |
0.45 | - |
succinate semialdehyde | with NAD+ as cofactor | Acinetobacter baylyi | |
0.87 | - |
NAD+ | - |
Acinetobacter baylyi | |
1.05 | - |
propanal | with NAD+ as cofactor | Acinetobacter baylyi | |
1.66 | - |
succinate semialdehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
3.9 | - |
acetaldehyde | with NADP+ as cofactor | Acinetobacter baylyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
2 * 62000, SDS-PAGE | Acinetobacter baylyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi | Q6FFQ0 | - |
- |
Pseudomonas putida | Q88GW5 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Acinetobacter baylyi |
to homogeneity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dioxopentanoate + NAD+ + H2O | - |
Acinetobacter baylyi | 2-oxoglutarate + NADH + H+ | - |
r | |
acetaldehyde + NAD(P)+ + H2O | - |
Acinetobacter baylyi | acetate + NAD(P)H + H+ | - |
? | |
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O | - |
Acinetobacter baylyi | alpha-ketoglutarate + NAD(P)H + H+ | - |
? | |
alpha-ketoglutaric semialdehyde + NADP+ + H2O | - |
Pseudomonas putida | alpha-ketoglutarate + NADPH + H+ | - |
? | |
glutaraldehyde + NAD(P)+ + H2O | - |
Acinetobacter baylyi | glutarate + NAD(P)H + H+ | - |
? | |
propanal + NAD(P)+ + H2O | - |
Acinetobacter baylyi | propionate + NAD(P)H + H+ | - |
? | |
succinate semialdehyde + NAD(P)+ + H2O | - |
Acinetobacter baylyi | ? + NAD(P)H + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 62000, SDS-PAGE | Acinetobacter baylyi |
Synonyms | Comment | Organism |
---|---|---|
alpha-ketoglutarate semialdehyde dehydrogenase | - |
Acinetobacter baylyi |
alpha-ketoglutarate semialdehyde dehydrogenase | - |
Pseudomonas putida |
alpha-KGSA dehydrogenase | - |
Acinetobacter baylyi |
alpha-KGSA dehydrogenase | - |
Pseudomonas putida |
alphaKGSADH | - |
Acinetobacter baylyi |
alphaKGSADH | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
succinate semialdehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
0.06 | - |
succinate semialdehyde | with NAD+ as cofactor | Acinetobacter baylyi | |
0.41 | - |
acetaldehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
0.58 | - |
propanal | with NADP+ as cofactor | Acinetobacter baylyi | |
0.65 | - |
Glutaraldehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
2.07 | - |
propanal | with NAD+ as cofactor | Acinetobacter baylyi | |
4.13 | - |
Glutaraldehyde | with NAD+ as cofactor | Acinetobacter baylyi | |
55 | - |
NADP+ | - |
Acinetobacter baylyi | |
57 | - |
NAD+ | - |
Acinetobacter baylyi | |
197 | - |
alpha-Ketoglutaric semialdehyde | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acinetobacter baylyi | |
NADP+ | - |
Pseudomonas putida | |
NADP+ | alpha-KGSA dehydrogenase is an NADP+-preferring enzyme, since it is 58times more efficient with NADP+ than with NAD+ | Acinetobacter baylyi |