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Literature summary for 1.2.1.27 extracted from
- Properties of purified methylmalonate semialdehyde dehydrogenase of Pseudomonas aeruginosa (1970), J. Biol. Chem., 245, 1828-1835.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
2-Methyl-3-oxopropanoate | - |
Pseudomonas aeruginosa |  |
| 0.022 | - |
CoA | - |
Pseudomonas aeruginosa | |
| 0.082 | - |
NAD+ | - |
Pseudomonas aeruginosa | |
| 0.54 | - |
2-mercaptoethanol | - |
Pseudomonas aeruginosa | |
| 6.1 | - |
propanal | - |
Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
2 * 58000, SDS-PAGE | Pseudomonas aeruginosa |
| 69000 | - |
2 * 69000, gel filtration in presence of SDS | Pseudomonas aeruginosa |
| 132000 | - |
gel filtration | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methyl-3-oxopropanoate + CoA + NAD+ | Pseudomonas aeruginosa | - |
propanoyl-CoA + CO2 + NADH | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methyl-3-oxopropanoate + CoA + NAD+ | - |
Pseudomonas aeruginosa | propanoyl-CoA + CO2 + NADH | - |
ir | |
| 2-methyl-3-oxopropanoate + CoA + NAD+ | other acyl acceptor: 2-mercaptoethanol | Pseudomonas aeruginosa | propanoyl-CoA + CO2 + NADH | - |
ir | |
| propanal + 2-mercaptoethanol + NAD+ | - |
Pseudomonas aeruginosa | ? + NADH | - |
ir | |
| propanal + CoA + NAD+ | - |
Pseudomonas aeruginosa | propanoyl-CoA + NADH | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 58000, SDS-PAGE | Pseudomonas aeruginosa |
| dimer | 2 * 69000, gel filtration in presence of SDS | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| CoA | - |
Pseudomonas aeruginosa | |
| NAD+ | - |
Pseudomonas aeruginosa | |
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Release 2023.1 (January 2023)
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