Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.27 extracted from

  • Norvienyeku, J.; Zhong, Z.; Lin, L.; Dang, X.; Chen, M.; Lin, X.; Zhang, H.; Anjago, W.M.; Lin, L.; Abdul, W.; Wang, Z.
    Methylmalonate-semialdehyde dehydrogenase mediated metabolite homeostasis essentially regulate conidiation, polarized germination and pathogenesis in Magnaporthe oryzae (2017), Environ. Microbiol., 19, 4256-4277 .
    View publication on PubMed
Search for more literature for 1.2.1.27

Cloned(Commentary)

Cloned (Comment) Organism
gene MGG_01606, single copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis Pyricularia oryzae

Protein Variants

Protein Variants Comment Organism
additional information DELTAMomsdh mutant, constructed by DNA targeted replacement of MoMSDH, produces only few restricted lesion on host tissues, MoMSDH deletion accounts for the drastic reduction in lesion size by rendering the DELTAMomsdh mutants incapable of mobilizing the necessary energy required to effectively colonize host tissues. DELTAMomsdh deletion mutants exhibit higher intracellular level of amino acids Val, Leu, Ile, and of pyridoxine Pyricularia oryzae

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde about 10% inhibition at 3 mM Pyricularia oryzae
DTT about 40% inhibition at 2 mM Pyricularia oryzae
ethanol complete inhibition at 3% v/v Pyricularia oryzae
H2O2 about 40% inhibition at 3 mM Pyricularia oryzae
malonate about 25% inhibition at 1 mM Pyricularia oryzae
NaCl about 40% inhibition at 0.7 mM Pyricularia oryzae
pyridoxine complete inhibition at 0.1 mg/ml Pyricularia oryzae
SDS about 50% inhibition at 0.01% Pyricularia oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Pyricularia oryzae
-
 propanoyl-CoA + HCO3- + NADH + H+
-
 ?
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Pyricularia oryzae FGSC 8958
-
 propanoyl-CoA + HCO3- + NADH + H+
-
 ?
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ Pyricularia oryzae ATCC MYA-4617
-
 propanoyl-CoA + HCO3- + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pyricularia oryzae G4MTY9 cv. Guy11, Pyricularia oryzae
-
Pyricularia oryzae ATCC MYA-4617 G4MTY9 cv. Guy11, Pyricularia oryzae
-
Pyricularia oryzae FGSC 8958 G4MTY9 cv. Guy11, Pyricularia oryzae
-

Source Tissue

Source Tissue Comment Organism Textmining
conidium
-
 Pyricularia oryzae
-
mycelium
-
 Pyricularia oryzae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
 Pyricularia oryzae propanoyl-CoA + HCO3- + NADH + H+
-
 ?
2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
 Pyricularia oryzae FGSC 8958 propanoyl-CoA + HCO3- + NADH + H+
-
 ?
2-methyl-3-oxopropanoate + CoA + H2O + NAD+
-
 Pyricularia oryzae ATCC MYA-4617 propanoyl-CoA + HCO3- + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
More MoMsdh domain architecture, overview Pyricularia oryzae

Synonyms

Synonyms Comment Organism
methylmalonate-semialdehyde dehydrogenase
-
 Pyricularia oryzae
MGG_01606
-
 Pyricularia oryzae
MoMSDH
-
 Pyricularia oryzae
MSDH
-
 Pyricularia oryzae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pyricularia oryzae

Expression

Organism Comment Expression
Pyricularia oryzae during infection, e.g. of susceptible rice cv. CO39 and seedlings of the barley cv. Golden Promise, two ALDH genes (MoMSDH and MoDEL) are exclusively upregulated at 48-hpi with MoMSDH showing about 5.8fold increase in expression pattern during the growth of the rice blast fungus in host tissue. The remaining eight Magnaporthe oryzae ALDH genes show low and unaltered expression pattern at all stages of infection up

General Information

General Information Comment Organism
evolution evolutionary lineage history of Msdh across kingdoms, MSDH belongs to the aldehyde dehydrogenase (ALDH) superfamily of genes which are highly conserved and widely distributed in almost all organisms across kingdoms. In vivo expression pattern of Magnaporthe oryzae specific ALDH genes during host-pathogen interaction, overview Pyricularia oryzae
malfunction MoMSDH deletion adversely affected the development of conidiophore and, as a result, conidiophore population as well as the number of conidia per conidiophore produced by the DELTAMomsdh deletion mutants are significantly reduced Pyricularia oryzae
metabolism contribution of the numerous ALDH genes to the fungal pathogenesis, 16 ALDH genes in Magnaporthe oryzae are idetified involved in infection and pathogenesis Pyricularia oryzae
physiological function enzyme MoMsdh exerts minimal influence on the development of vegetative hyphae, but is involved in the regulation of conidiogenesis and conidiophoregenesis in Magnaporthe oryzae. Conidiation constitutes a pivotal developmental stage in fungal life-cycle and represents one of the most durable organs that promote fungal survival and facilitate their efficient dissemination. MoMsdh is involved in the regulating conditions necessary for promoting asexual reproductive development in Magnaporthe oryzae. MoMsdh is essential for pathogenicity of the organism. MoMsdh crucially regulates intracellular level of small branched-chain amino acids and appressoria mediating signalling molecules. MoMsdh specifically controls the localization of Spitzenkoerper in the conidium to ensure polarized speciation of germ tube. MoMsdh enhances membrane integrity by detoxifying alcohol and pyridoxine derived reactive osmolytes Pyricularia oryzae