General Stability | Organism |
---|---|
dithiothreitol stabilizes | Acinetobacter calcoaceticus |
MgCl2 stabilizes | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00057 | - |
benzaldehyde | - |
Acinetobacter calcoaceticus | |
0.079 | - |
NAD+ | - |
Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, dehydrogenase II, SDS-PAGE | Acinetobacter calcoaceticus |
225000 | - |
dehydrogenase II, gel filtration | Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | Acinetobacter calcoaceticus | - |
benzoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
dehydrogenase II | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
79 | - |
- |
Acinetobacter calcoaceticus |
Storage Stability | Organism |
---|---|
-20°C, 100 mM potassium phosphate, pH 7.5, 2 mM dithiothreitol, 1 mM EDTA, 1 mM benzamidine, 1.2 mM phenylmethanesulfonylfluoride, 40% glycerol, 3 months, 10% loss of activity | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | - |
Acinetobacter calcoaceticus | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Acinetobacter calcoaceticus | benzoate + NADH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, dehydrogenase II, SDS-PAGE | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acinetobacter calcoaceticus |