Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be used for production of bio-vanillin from vanillic acid | Trametes cinnabarina |
Cloned (Comment) | Organism |
---|---|
gene PcALDH, cloning from RNA via RT-PCR, sequence comparisons and phylogenetic analysis, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain JM109 | Trametes cinnabarina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | enzyme PcALDH contains a signal peptide | Trametes cinnabarina | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
vanillin + NAD+ | Trametes cinnabarina | - |
vanillinic acid + NADH + H+ | - |
r | |
vanillin + NAD+ | Trametes cinnabarina MUCL 39533 | - |
vanillinic acid + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes cinnabarina | A0A0U2ETT9 | - |
- |
Trametes cinnabarina MUCL 39533 | A0A0U2ETT9 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aromatic aldehyde + NAD+ + H2O = an aromatic acid + NADH + H+ | ALDH catalysis involves acylation and deacylation | Trametes cinnabarina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Trametes cinnabarina | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues | Trametes cinnabarina | ? | - |
? | |
additional information | ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues | Trametes cinnabarina MUCL 39533 | ? | - |
? | |
vanillin + NAD+ | - |
Trametes cinnabarina | vanillinic acid + NADH + H+ | - |
r | |
vanillin + NAD+ | - |
Trametes cinnabarina MUCL 39533 | vanillinic acid + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PcALDH | - |
Trametes cinnabarina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Trametes cinnabarina | |
NADH | - |
Trametes cinnabarina |
General Information | Comment | Organism |
---|---|---|
evolution | in silico analysis of PcALDH indicate that enzyme PcALDH belongs to the ALDH superfamily and class 3 ALDHs | Trametes cinnabarina |
metabolism | in filamentous fungi, vanillin is formed in a two-stage process in which ferulic acid is converted to vanillic acid then reduced to vanillin | Trametes cinnabarina |
physiological function | reduction of vanillic acid to vanillin is catalysed by the key enzyme aryl-aldehyde dehydrogenase | Trametes cinnabarina |