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Literature summary for 1.2.1.3 extracted from
- Purification and characterization of two aldehyde dehydrogenases from Pseudomonas aeruginosa (1977), Eur. J. Biochem., 81, 185-192.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | slight activation | Pseudomonas aeruginosa |  |
| NH4+ | activation seems to be due to increase of ionic strength | Pseudomonas aeruginosa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Iodosobenzoate | 0.1 mM, 3 min, complete inhibition | Pseudomonas aeruginosa | |
| p-hydroxymercuribenzoate | 0.1 mM, 3 min, complete inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | influence of pH-value and buffer on Km-value for hexanal | Pseudomonas aeruginosa | |
| 0.0095 | - |
hexanal | - |
Pseudomonas aeruginosa | |
| 0.045 | - |
Butanal | - |
Pseudomonas aeruginosa | |
| 0.064 | - |
propanal | - |
Pseudomonas aeruginosa | |
| 0.165 | - |
acetaldehyde | - |
Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | slight activation | Pseudomonas aeruginosa | |
| K+ | activation seems to be due to increase of ionic strength | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 225000 | - |
gel filtration | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas aeruginosa | constitutive enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -8°C, 16% glycerol, 1 mM dithiothreitol, 25% loss of activity after 6 months | Pseudomonas aeruginosa |
| 4°C, 60% loss of activity after 44 h in absence of glycerol, 10% loss of activity in presence of 16% glycerol | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NAD+ + H2O | - |
Pseudomonas aeruginosa | acetate + NADH + H+ | - |
? | |
| butanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | butyrate + NADH + H+ | - |
? | |
| decanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | decanoate + NADH | - |
? | |
| hexanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | hexanoate + NADH + H+ | - |
? | |
| additional information | constitutive enzyme | Pseudomonas aeruginosa | ? | - |
? | |
| octanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | octanoate + NADH | - |
? | |
| propanal + NAD+ + H2O | - |
Pseudomonas aeruginosa | propionate + NADH | - |
? | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
30°C, stable | Pseudomonas aeruginosa |
| 8.6 | - |
30°C, rapid inactivation, NAD+ protects | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas aeruginosa | |
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