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Literature summary for 1.2.1.3 extracted from
- Structural and functional consequences of coenzyme binding to the inactive Asian variant of mitochondrial aldehyde dehydrogenase: roles of residues 475 and 487 (2007), J. Biol. Chem., 282, 12940-12950.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapour diffusion method with 100 mM ACES, pH 6.6-6.8, 5-10 mM MgCl2, 100 mM guanidine HCl, 15% (w/v) PEG 6000, and 4-8 mM dithiothreitol | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K487E | reduced activity | Homo sapiens |
| R475Q | the mutant is less thermally stable by 15°C and shows 2fold reduction in kcat-value compared to the wild type enzyme | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P05091 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALDH2 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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