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Literature summary for 1.2.1.3 extracted from

  • Raj, S.; Rathnasingh, C.; Jung, W.; Selvakumar, E.; Park, S.
    A novel NAD+-dependent aldehyde dehydrogenase encoded by the puuC gene of Klebsiella pneumoniae DSM 2026 that utilizes 3-hydroxypropionaldehyde as a substrate (2010), Biotechnol. Bioprocess Eng., 15, 131-138.
No PubMed abstract available
Search for more literature for 1.2.1.3

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol 161.09% activity at 1 mM Klebsiella pneumoniae
dithiothreitol 147.48% activity at 1 mM Klebsiella pneumoniae
Semicarbazide hydrochloride 118.98% activity at 1 mM Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells (strain SH-KAD2) Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Klebsiella pneumoniae
Co2+ 89.01% residual activity at 1 mM Klebsiella pneumoniae
Cu2+ complete inhibition at 1 mM Klebsiella pneumoniae
Fe2+ 26.34% residual activity at 1 mM Klebsiella pneumoniae
Hg2+ complete inhibition at 1 mM Klebsiella pneumoniae
Li+ 90.64% residual activity at 1 mM Klebsiella pneumoniae
Mg2+ 93.64% residual activity at 1 mM Klebsiella pneumoniae
Na+ 89.17% residual activity at 1 mM Klebsiella pneumoniae
NH3 92.06% residual activity at 1 mM Klebsiella pneumoniae
Ni2+ 89.54% residual activity at 1 mM Klebsiella pneumoniae
Zn2+ 35.91% residual activity at 1 mM Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 NAD+ recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae
0.14
-
 Valeraldehyde recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae
0.21
-
 Isovaleraldehyde recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae
0.26
-
 Butyraldehyde recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae
0.48
-
 3-hydroxypropionaldehyde recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 recombinant enzyme, SDS-PAGE Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae DSM 2026
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13
-
 crude extract, using 3-hydroxypropionaldehyde as substrate, in 50 mM, potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae
27.73
-
 after 8.86fold purification, using 3-hydroxypropionaldehyde as substrate, in 50 mM, potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-furaldehyde + NAD+ + H2O the activity on 2-furaldehyde is 22% of that on 3-hydroxypropionaldehyde Klebsiella pneumoniae 2-furanoic acid + NADH + H+
-
 ?
3-hydroxypropionaldehyde + NAD+ + H2O the activity on 3-hydroxypropionaldehyde is about 70% of that on valeraldehyde Klebsiella pneumoniae 3-hydroxypropanoate + NADH + 2 H+
-
 ?
acetaldehyde + NAD+ + H2O the activity on acetaldehyde is about 50% of that on valeraldehyde Klebsiella pneumoniae acetate + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O the activity on acetaldehyde is about 50% of that on valeraldehyde Klebsiella pneumoniae DSM 2026 acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O the activity on benzaldehyde is 35% of that on 3-hydroxypropionaldehyde Klebsiella pneumoniae benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O the activity on benzaldehyde is 35% of that on 3-hydroxypropionaldehyde Klebsiella pneumoniae DSM 2026 benzoate + NADH + H+
-
 ?
butyraldehyde + NAD+ + H2O the activity on butyraldehyde is about 95% of that on valeraldehyde Klebsiella pneumoniae butanoate + NADH + H+
-
 ?
isovaleraldehyde + NAD+ + H2O the activity on isovaleraldehyde is about 80% of that on valeraldehyde Klebsiella pneumoniae isovalerate + NADH + H+
-
 ?
additional information no appreciable activity is observed on formaldehyde Klebsiella pneumoniae ?
-
 ?
additional information no appreciable activity is observed on formaldehyde Klebsiella pneumoniae DSM 2026 ?
-
 ?
propionaldehyde + NAD+ + H2O the activity on propionaldehyde is about 55% of that on valeraldehyde Klebsiella pneumoniae propionate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O the activity on propionaldehyde is about 55% of that on valeraldehyde Klebsiella pneumoniae DSM 2026 propionate + NADH + H+
-
 ?
valeraldehyde + NAD+ + H2O most preferred substrate, 100% activity Klebsiella pneumoniae valerate + NADH + H+
-
 ?
valeraldehyde + NAD+ + H2O most preferred substrate, 100% activity Klebsiella pneumoniae DSM 2026 valerate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
ALDH
-
 Klebsiella pneumoniae
NAD+-dependent aldehyde dehydrogenase
-
 Klebsiella pneumoniae
NAD+-specific ALDH
-
 Klebsiella pneumoniae
PuuC
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme is stable between 30°C and 50°C, but becomes unstable at 60°C Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
 Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 the enzyme activity is completely abolished at pH 6.0 Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is the preferred coenzyme over NADP+ Klebsiella pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
41.44
-
 3-hydroxypropionaldehyde recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae
117.2
-
 Butyraldehyde recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae
121.9
-
 Isovaleraldehyde recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae
207.1
-
 Valeraldehyde recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae
254.7
-
 NAD+ recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C Klebsiella pneumoniae