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Literature summary for 1.2.1.30 extracted from

  • Li, T.; Rosazza, J.P.N.
    Purification, characterization, and properties of an aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 (1997), J. Bacteriol., 179, 3482-3487.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.30

General Stability

General Stability Organism
glycerol in chromatographic buffers stabilizes Nocadia sp.

Inhibitors

Inhibitors Comment Organism Structure
anthranilic acid competitive inhibitor with benzoic acid as a substrate Nocadia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0293
-
 NADPH
-
 Nocadia sp.
0.0345
-
 (R)-ibuprofen
-
 Nocadia sp.
0.0573
-
 ATP
-
 Nocadia sp.
0.155
-
 Ibuprofen
-
 Nocadia sp.
0.645
-
 benzoate
-
 Nocadia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 1 * 140000, SDS-PAGE Nocadia sp.
163000
-
 gel filtration Nocadia sp.

Organism

Organism UniProt Comment Textmining
Nocadia sp.
-
-
-
Nocadia sp. NRRL 5646
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by a combination of Mono-Q, Reactive Green 19 agarose affinity chromatography, and hydroxyapatite chromatography Nocadia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.89
-
 purified enzyme Nocadia sp.

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl buffer, pH 7.5, 10% glycerol, 8 hours Nocadia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-ibuprofen + NADPH + ATP
-
 Nocadia sp. (2R)-2-(4-(2-methylpropyl)phenyl)propanal + NADP+ + AMP + H2O
-
 ?
(R)-ibuprofen + NADPH + ATP
-
 Nocadia sp. NRRL 5646 (2R)-2-(4-(2-methylpropyl)phenyl)propanal + NADP+ + AMP + H2O
-
 ?
aromatic acids + NADPH + H+ + ATP
-
 Nocadia sp. aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 ir
aromatic acids + NADPH + H+ + ATP
-
 Nocadia sp. NRRL 5646 aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 ir
benzoic acid + NADPH + H+ + ATP the reaction proceeds via benzoyladenosine 5'-monophosphate which is further reduced by NADPH to benzaldehyde Nocadia sp. benzaldehyde + NADP+ + AMP + diphosphate
-
 ?
benzoic acid + NADPH + H+ + ATP the reaction proceeds via benzoyladenosine 5'-monophosphate which is further reduced by NADPH to benzaldehyde Nocadia sp. NRRL 5646 benzaldehyde + NADP+ + AMP + diphosphate
-
 ?
ibuprofen + NADPH + ATP
-
 Nocadia sp. 2-(4-isobutylphenyl)propanal + NADP+ + AMP + H2O
-
 ?
ibuprofen + NADPH + ATP
-
 Nocadia sp. NRRL 5646 2-(4-isobutylphenyl)propanal + NADP+ + AMP + H2O
-
 ?
additional information other substrates are phenyl-substituted aliphatic acids, heterocyclic carboxylic acids, polyaromatic ring carboxylic acids, ibuprofen and its (R)-(-) isomer Nocadia sp. ?
-
 ?
additional information other substrates are phenyl-substituted aliphatic acids, heterocyclic carboxylic acids, polyaromatic ring carboxylic acids, ibuprofen and its (R)-(-) isomer Nocadia sp. NRRL 5646 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 140000, SDS-PAGE Nocadia sp.

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Nocadia sp.
NADPH
-
 Nocadia sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.261
-
 anthranilic acid
-
 Nocadia sp.