Cloned (Comment) | Organism |
---|---|
encoded by two different genes, LYS2 and LYS5 | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
addition of 10 mM 2-mercaptoethanol and protease inhibitors e.g.: 5 mM EDTA, 1 mM phenylmethylsulfonyl fluoride to the crude extract improves stability | Saccharomyces cerevisiae |
low ionic strength: unstable | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alpha-aminoadipate + NAD(P)H | Saccharomyces cerevisiae | in presence of ATP and Mg2+ | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | Saccharomyces cerevisiae | sixth step of lysine biosynthesis | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, using column chromatography on DEAE-cellulose, gel filtration, hydrolxylapatite and Reactive Red-120 agarose | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminoadipate + NADPH | in presence of ATP + Mg2+ | Saccharomyces cerevisiae | L-2-aminoadipate 6-semialdehyde + NADP+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | in presence of ATP and Mg2+ | Saccharomyces cerevisiae | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | sixth step of lysine biosynthesis | Saccharomyces cerevisiae | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-70 | - |
unstable | Saccharomyces cerevisiae |
-20 | - |
unstable | Saccharomyces cerevisiae |
4 | - |
unstable | Saccharomyces cerevisiae |
30 | - |
unstable | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae | |
NADPH | - |
Saccharomyces cerevisiae |