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Literature summary for 1.2.1.31 extracted from

  • Storts, D.R.; Bhattacharjee, J.K.
    Properties of revertants of lys2 and lys5 mutants as well as alpha-aminoadipate-semialdehyde dehydrogenase from Saccharomyces cerevisiae (1989), Biochem. Biophys. Res. Commun., 161, 182-186.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
encoded by two different genes, LYS2 and LYS5 Saccharomyces cerevisiae

General Stability

General Stability Organism
addition of 10 mM 2-mercaptoethanol and protease inhibitors e.g.: 5 mM EDTA, 1 mM phenylmethylsulfonyl fluoride to the crude extract improves stability Saccharomyces cerevisiae
low ionic strength: unstable Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alpha-aminoadipate + NAD(P)H Saccharomyces cerevisiae in presence of ATP and Mg2+ L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
L-alpha-aminoadipate + NAD(P)H Saccharomyces cerevisiae sixth step of lysine biosynthesis L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using column chromatography on DEAE-cellulose, gel filtration, hydrolxylapatite and Reactive Red-120 agarose Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminoadipate + NADPH in presence of ATP + Mg2+ Saccharomyces cerevisiae L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
?
L-alpha-aminoadipate + NAD(P)H in presence of ATP and Mg2+ Saccharomyces cerevisiae L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?
L-alpha-aminoadipate + NAD(P)H sixth step of lysine biosynthesis Saccharomyces cerevisiae L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-70
-
unstable Saccharomyces cerevisiae
-20
-
unstable Saccharomyces cerevisiae
4
-
unstable Saccharomyces cerevisiae
30
-
unstable Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae
NADPH
-
Saccharomyces cerevisiae