Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Candida maltosa | |
Fe2+ | - |
Candida maltosa | |
glutamate | competitive inhibitor with respect to alpha-aminoadipate | Candida maltosa | |
Hg2+ | - |
Candida maltosa | |
L-aspartate | competitive inhibitor with respect to alpha-aminoadipate | Candida maltosa | |
Sn2+ | - |
Candida maltosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NADPH | - |
Candida maltosa | |
0.2 | - |
alpha-aminoadipate | - |
Candida maltosa | |
0.38 | - |
NADH | - |
Candida maltosa | |
0.5 | - |
ATP | - |
Candida maltosa | |
3.8 | - |
MgCl2 | - |
Candida maltosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulates | Candida maltosa | |
Cu2+ | inhibits | Candida maltosa | |
Mg2+ | stimulates | Candida maltosa | |
Mg2+ | required for activity | Candida maltosa | |
Mn2+ | stimulates | Candida maltosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
gel filtration | Candida maltosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alpha-aminoadipate + NAD(P)H | Candida maltosa | in presence of ATP and Mg2+ | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | Candida maltosa | involved in lysine biosynthetic pathway | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | Candida maltosa L4 | in presence of ATP and Mg2+ | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | Candida maltosa L4 | involved in lysine biosynthetic pathway | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida maltosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using column chromatography on Sephadex G-25 and Sephadex G-200 | Candida maltosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0063 | - |
5 mM L-lysine as supplement during growth of the enzyme | Candida maltosa |
0.0072 | - |
no supplement added during growth of the enzyme | Candida maltosa |
0.0138 | - |
10 mM amitrole as supplement during growth of the enzyme | Candida maltosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-aminoadipic semialdehyde + NAD+ + H2O | - |
Candida maltosa | alpha-aminoadipic acid + NADH + H+ | - |
? | |
alpha-aminoadipic semialdehyde + NAD+ + H2O | - |
Candida maltosa L4 | alpha-aminoadipic acid + NADH + H+ | - |
? | |
L-2-aminoadipate + NADPH | in presence of ATP + Mg2+ | Candida maltosa | L-2-aminoadipate 6-semialdehyde + NADP+ + H2O | - |
? | |
L-2-aminoadipate + NADPH | in presence of ATP + Mg2+ | Candida maltosa L4 | L-2-aminoadipate 6-semialdehyde + NADP+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | in presence of ATP and Mg2+ | Candida maltosa | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | involved in lysine biosynthetic pathway | Candida maltosa | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | in presence of ATP and Mg2+ | Candida maltosa L4 | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | involved in lysine biosynthetic pathway | Candida maltosa L4 | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Candida maltosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | can replace NADPH with lower activity and higher Km | Candida maltosa | |
NADPH | - |
Candida maltosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
L-glutamate | - |
Candida maltosa | |
4.2 | - |
L-aspartate | - |
Candida maltosa |