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Literature summary for 1.2.1.31 extracted from
- Domain structure characterization of the multifunctional alpha-aminoadipate reductase from Penicillium chrysogenum by limited proteolysis. Activation of alpha-aminoadipate does not require the peptidyl carrier protein box or the reduction domain (2003), J. Biol. Chem., 278, 8250-8256.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | presence of ATP required | Penicillium chrysogenum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, 75% inhibition | Penicillium chrysogenum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
NADPH | pH 8, 30°C | Penicillium chrysogenum | |
| 1.3 | - |
ATP | pH 8, 30°C | Penicillium chrysogenum | |
| 1.4 | - |
L-2-aminoadipate | pH 8, 30°C | Penicillium chrysogenum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolue requiremnt, optimal concentration: 10 mM | Penicillium chrysogenum | |
| Mn2+ | optimal concentration: 5 mM, 60% of the maximal activity attained with Mg2+ | Penicillium chrysogenum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 155000 | - |
1 * 155000, SDS-PAGE | Penicillium chrysogenum |
| 171000 | - |
gel filtration | Penicillium chrysogenum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Penicillium chrysogenum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Penicillium chrysogenum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% v/v glycerol, rapid loss of activity | Penicillium chrysogenum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-2-aminoadipate + NADPH + H+ | presence of ATP required | Penicillium chrysogenum | L-2-aminoadipate 6-semialdehyde + NADP+ + H2O | - |
? | |
| S-carboxymethyl-L-cysteine + NADPH | 5% of the activity with L-2-aminoadipate | Penicillium chrysogenum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 155000, SDS-PAGE | Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 29 | - |
Penicillium chrysogenum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
1 h, stable | Penicillium chrysogenum |
| 30 | - |
1 h, 20% loss of activity | Penicillium chrysogenum |
| 37 | - |
15 min, 50% loss of activity | Penicillium chrysogenum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 66 | - |
L-2-aminoadipate | pH 8, 30°C | Penicillium chrysogenum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Penicillium chrysogenum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Penicillium chrysogenum | |
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