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Literature summary for 1.2.1.32 extracted from

  • Davis, I.; Yang, Y.; Wherritt, D.; Liu, A.
    Reassignment of the human aldehyde dehydrogenase ALDH8A1 (ALDH12) to the kynurenine pathway in tryptophan catabolism (2018), J. Biol. Chem., 293, 9594-9603 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
N155A the mutant shows very low activity compared to the wild type enzyme Homo sapiens
N155D the mutant shows very low activity compared to the wild type enzyme Homo sapiens
N155Q the mutant shows very low activity compared to the wild type enzyme Homo sapiens
N169A inactive Homo sapiens
N169D inactive Homo sapiens
N169Q inactive Homo sapiens
R109A the variant has a similar turnover number but approximately 160fold increased Km as compared with wild type enzyme Homo sapiens
R451A inactive Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00059
-
 2-aminomuconate 6-semialdehyde wild type enzyme, pH and temperature not specified in the publication Homo sapiens
0.097
-
 2-aminomuconate 6-semialdehyde mutant enzyme R109A, pH and temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminomuconate 6-semialdehyde + NAD+ + H2O Homo sapiens
-
 2-aminomuconate + NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H2A2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000031
-
 mutant enzyme N155Q, pH and temperature not specified in the publication Homo sapiens
0.0000074
-
 mutant enzyme N155A, pH and temperature not specified in the publication Homo sapiens
0.0000074
-
 mutant enzyme N155D, pH and temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminomuconate 6-semialdehyde + NAD+ + H2O
-
 Homo sapiens 2-aminomuconate + NADH + 2 H+
-
 ?
2-hydroxymuconate 6-semialdehyde + NAD+ + H2O
-
 Homo sapiens 2-hydroxymuconate + NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
2-aminomuconate semialdehyde dehydrogenase
-
 Homo sapiens
2-AMS dehydrogenase
-
 Homo sapiens
aldehyde dehydrogenase
-
 Homo sapiens
ALDH12
-
 Homo sapiens
Aldh8a1
-
 Homo sapiens
AMSDH
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.42
-
 2-aminomuconate 6-semialdehyde wild type enzyme, pH and temperature not specified in the publication Homo sapiens
1.06
-
 2-aminomuconate 6-semialdehyde mutant enzyme R109A, pH and temperature not specified in the publication Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme is the aldehyde dehydrogenase of the kynurenine pathway of tryptophan catabolism Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11
-
 2-aminomuconate 6-semialdehyde mutant enzyme R109A, pH and temperature not specified in the publication Homo sapiens
710
-
 2-aminomuconate 6-semialdehyde wild type enzyme, pH and temperature not specified in the publication Homo sapiens