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Literature summary for 1.2.1.36 extracted from
- Characterization of retinaldehyde dehydrogenase 3 (2006), Biochem. J., 394, 67-75.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the cloning vector pGEM-T Easy and subcloned into the expression vector pET14b | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F471L | mutant | Mus musculus |
| T1411C | mutant | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,3',5-tri-iodothyronine | - |
Mus musculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
all-trans-retinal | wild-type | Mus musculus | |
| 0.0002 | - |
all-trans-retinal | F471L mutant | Mus musculus | |
| 0.0007 | - |
octanal | wild-type | Mus musculus | |
| 0.0009 | - |
octanal | F471L mutant | Mus musculus | |
| 0.0065 | - |
decanal | wild-type | Mus musculus | |
| 0.0221 | - |
hexanal | wild-type | Mus musculus | |
| 0.0431 | - |
NAD+ | F471L mutant | Mus musculus | |
| 0.0528 | - |
NAD+ | wild-type | Mus musculus | |
| 0.103 | - |
benzaldehyde | wild-type | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| retinal + NAD+ + H2O | Mus musculus | - |
retinoate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q9JHW9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a nickel-chelating Sepharose and a S300 HR gel-filtration column | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzaldehyde + NAD+ + H2O | - |
Mus musculus | benzoate + NADH + H+ | - |
? | |
| decanal + NAD+ + H2O | - |
Mus musculus | decanoate + NADH + H+ | - |
? | |
| hexanal + NAD+ + H2O | - |
Mus musculus | hexanoate + NADH + H+ | - |
? | |
| octanal + NAD+ + H2O | - |
Mus musculus | octanoate + NADH + H+ | - |
? | |
| retinal + NAD+ + H2O | - |
Mus musculus | retinoate + NADH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RALDH3 | - |
Mus musculus |
| Retinal dehydrogenase | - |
Mus musculus |
| retinaldehyde dehydrogenase 3 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
benzaldehyde | wild-type | Mus musculus | |
| 0.3 | - |
hexanal | wild-type | Mus musculus | |
| 0.4 | - |
decanal | wild-type | Mus musculus | |
| 3.1 | - |
NAD+ | F471L mutant | Mus musculus | |
| 3.3 | - |
NAD+ | wild-type | Mus musculus | |
| 3.9 | - |
retinal | all-trans-retinal, wild-type | Mus musculus | |
| 4.8 | - |
octanal | wild-type | Mus musculus | |
| 5.4 | - |
octanal | F471L mutant | Mus musculus | |
| 13 | - |
retinal | all-trans-retinal, F471L mutant | Mus musculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
activity assay | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Mus musculus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0377 | - |
3,3',5-tri-iodothyronine | - |
Mus musculus | |
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