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Literature summary for 1.2.1.39 extracted from

  • Fujioka, M.; Morino, Y.; Wada, H.
    Metabolism of phenylalanine (Achromobacter eurydice) (1970), Methods Enzymol., 17A, 585-596.
No PubMed abstract available
Search for more literature for 1.2.1.39

General Stability

General Stability Organism
unstable after dilution Achromobacter eurydice

Inhibitors

Inhibitors Comment Organism Structure
K+ above 1 M Achromobacter eurydice
N-ethylmaleimide
-
 Achromobacter eurydice
p-chloromercuribenzoate
-
 Achromobacter eurydice

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 NAD+
-
 Achromobacter eurydice

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ monovalent cation required. K+ , Rb+ , Na+, Li+, NH4+ and Cs+ activate in this order Achromobacter eurydice
K+ monovalent cation required. K+ , Rb+ , Na+, Li+, NH4+ and Cs+ activate in this order Achromobacter eurydice
Li+ monovalent cation required. K+ , Rb+ , Na+, Li+, NH4+ and Cs+ activate in this order Achromobacter eurydice
additional information divalent cations (Mg2+, Ca2+) are inactive Achromobacter eurydice
Na+ monovalent cation required. K+ , Rb+ , Na+, Li+, NH4+ and Cs+ activate in this order Achromobacter eurydice
NH4+ monovalent cation required. K+ , Rb+ , Na+, Li+, NH4+ and Cs+ activate in this order Achromobacter eurydice
Rb+ monovalent cation required. K+ , Rb+ , Na+, Li+, NH4+ and Cs+ activate in this order Achromobacter eurydice

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetaldehyde + NAD+ + H2O Achromobacter eurydice inducible enzyme appears when cells are grown on L-phenylalanine or on L-tryptophan as sole source of carbon ?
-
 ?

Organism

Organism UniProt Comment Textmining
Achromobacter eurydice
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Achromobacter eurydice

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19
-
-
 Achromobacter eurydice

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indoleacetaldehyde + NAD+ + H2O slow reaction Achromobacter eurydice indoleacetate + NADH
-
 ?
n-butyraldehyde + NAD+ + H2O slow reaction Achromobacter eurydice n-butyrate + NADH
-
 ?
phenylacetaldehyde + NAD+ + H2O highly specific for phenylacetaldehyde, NADP+ is about 1% as active as NAD+ Achromobacter eurydice phenylacetate + NADH + H+
-
 ir
phenylacetaldehyde + NAD+ + H2O inducible enzyme appears when cells are grown on L-phenylalanine or on L-tryptophan as sole source of carbon Achromobacter eurydice ?
-
 ?
propionaldehyde + NAD+ + H2O slow reaction Achromobacter eurydice propionate + NADH
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.9
-
-
 Achromobacter eurydice

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 unstable after dilution, especially in alkaline medium Achromobacter eurydice

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+ is about 1% as active as NAD+ Achromobacter eurydice