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Literature summary for 1.2.1.39 extracted from

  • Zimmerling, J.; Tischler, D.; Grossmann, C.; Schloemann, M.; Oelschlaegel, M.
    Characterization of aldehyde dehydrogenases applying an enzyme assay with in situ formation of phenylacetaldehydes (2017), Appl. Biochem. Biotechnol., 182, 1095-1107 .
    View publication on PubMed
Search for more literature for 1.2.1.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 phenylacetaldehyde with NAD+ as cosubstrate, at pH, temperature not specified in the publication Escherichia coli
0.5
-
 NAD+ with phenylacetaldehyde as cosubstrate, at pH 7.7, temperature not specified in the publication Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetaldehyde + NAD+ + H2O Escherichia coli
-
 phenylacetate + NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P80668
-
-

Storage Stability

Storage Stability Organism
-20°C, in 250 mM Tris-HCl pH 7.0, 3 months, 52% loss of activity Escherichia coli
0°C, in 250 mM Tris-HCl pH 7.0, 3 months, 46% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chlorophenylacetaldehyde + NAD+ + H2O high activity Escherichia coli 4-chlorophenylacetate + NADH + 2 H+
-
 ?
4-fluorophenylacetaldehyde + NAD+ + H2O high activity Escherichia coli 4-fluorophenylacetate + NADH + 2 H+
-
 ?
additional information no activity with phenazine methosulfate Escherichia coli ?
-
-
phenylacetaldehyde + NAD+ + H2O
-
 Escherichia coli phenylacetate + NADH + 2 H+
-
 ?
phenylacetaldehyde + NAD+ + H2O highest activity Escherichia coli phenylacetate + NADH + 2 H+
-
 ?
phenylacetaldehyde + NADP+ + H2O 11% activity compared to NAD+ Escherichia coli phenylacetate + NADPH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
feaB
-
 Escherichia coli
NAD(P)+-dependent phenylacetaldehyde dehydrogenase
-
 Escherichia coli
PAD
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 55 after preincubation of the protein for 30 min at 0 to 45°C, residual activities of 82.7 to 100% are measured. After the incubation for 30 min at 50°C, this enzyme shows an activity of about 63.1%, whereas significant enzyme inactivation is observed after incubation at 55°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+ the most suitable co-substrate is NAD+ Escherichia coli