Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A200E | the mutant shows strongly reduced activity (50fold) with NADP+ compared to the wild type enzyme | Saccharomyces cerevisiae |
A200E/V210Q | the mutant shows strongly reduced activity (250fold) with NADP+ and about 3fold increased activity with NAD+ compared to the wild type enzyme | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADP+ | Saccharomyces cerevisiae | - |
acetate + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00312 | - |
native enzyme, with NAD+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
0.00315 | - |
mutant enzyme A200E, with NAD+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
0.0113 | - |
mutant enzyme A200E/V210Q, with NAD+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
0.012 | - |
mutant enzyme A200E/V210Q, with NADP+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
0.06 | - |
mutant enzyme A200E, with NADP+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
3 | - |
native enzyme, with NADP+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADP+ | - |
Saccharomyces cerevisiae | acetate + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALDH1 | - |
Saccharomyces cerevisiae |
NADP dependent aldehyde dehydrogenase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | preferred cofactor | Saccharomyces cerevisiae |