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Literature summary for 1.2.1.42 extracted from

  • Lee, C.Y.; Meighen, E.A.
    Cysteine-286 as the site of acylation of the lux-specific fatty acyl-CoA reductase (1997), Biochim. Biophys. Acta, 1338, 215-222.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Photobacterium phosphoreum
expression in Escherichia coli Photobacterium leiognathi

Protein Variants

Protein Variants Comment Organism
C171S 77% of wild-type activity Photobacterium phosphoreum
C171S 99% of wild-type activity Photobacterium leiognathi
C279S 90% of wild-type activity Photobacterium phosphoreum
C279S 79% of wild -type activity Photobacterium leiognathi
C286S 9% of wild-type activity Photobacterium phosphoreum
C286S 2% of wild-type activity Photobacterium leiognathi

Organism

Organism UniProt Comment Textmining
Photobacterium leiognathi
-
-
-
Photobacterium phosphoreum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
20.0 light emission units/mg after 4 min incubation in luciferase coupled assay Photobacterium leiognathi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hexadecanoyl-CoA + NAD(P)H
-
Photobacterium phosphoreum hexadecanal + NAD(P)+
-
?
hexadecanoyl-CoA + NAD(P)H
-
Photobacterium leiognathi hexadecanal + NAD(P)+
-
?

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
Photobacterium phosphoreum
NAD(P)H
-
Photobacterium leiognathi