Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.42 extracted from

  • Doan, T.T.; Domergue, F.; Fournier, A.E.; Vishwanath, S.J.; Rowland, O.; Moreau, P.; Wood, C.C.; Carlsson, A.S.; Hamberg, M.; Hofvander, P.
    Biochemical characterization of a chloroplast localized fatty acid reductase from Arabidopsis thaliana (2012), Biochim. Biophys. Acta, 1821, 1244-1255.
    View publication on PubMed
Search for more literature for 1.2.1.42

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain W303-1A and in Nicotiana benthamiana leaves Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00146
-
 [hexadecanoyl]-acyl carrier protein in 100 mM phosphate buffer, pH 7.0, at 30°C Arabidopsis thaliana
0.0036
-
 hexadecanoyl-CoA in 100 mM phosphate buffer, pH 7.0, at 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
heptadecanoyl-CoA + NADPH + H+ Arabidopsis thaliana
-
 heptadecanal + CoA + NADP+
-
 ?
hexadecanoyl-CoA + NADPH + H+ Arabidopsis thaliana preferred substrate hexadecanal + CoA + NADP+
-
 ?
additional information Arabidopsis thaliana no products are detected when using C18:1-CoA, 2-methyl-16:0-CoA, 2-methyl-18:0-CoA or C16:0 free fatty acid as substrates ?
-
 ?
octadecanoyl-CoA + NADPH + H+ Arabidopsis thaliana low activity octadecanal + CoA + NADP+
-
 ?
[hexadecanoyl]-acyl carrier protein + NADPH + H+ Arabidopsis thaliana preferred substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Ni2+ resin column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heptadecanoyl-CoA + NADPH + H+
-
 Arabidopsis thaliana heptadecanal + CoA + NADP+
-
 ?
hexadecanoyl-CoA + NADPH + H+ preferred substrate Arabidopsis thaliana hexadecanal + CoA + NADP+
-
 ?
additional information no products are detected when using C18:1-CoA, 2-methyl-16:0-CoA, 2-methyl-18:0-CoA or C16:0 free fatty acid as substrates Arabidopsis thaliana ?
-
 ?
octadecanoyl-CoA + NADPH + H+ low activity Arabidopsis thaliana octadecanal + CoA + NADP+
-
 ?
[hexadecanoyl]-acyl carrier protein + NADPH + H+ preferred substrate Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
FAR6
-
 Arabidopsis thaliana
fatty acid reductase
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Arabidopsis thaliana