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Literature summary for 1.2.1.48 extracted from

  • Lloyd, M.D.; Boardman, K.D.; Smith, A.; van den Brink, D.M.; Wanders, R.J.; Threadgill, M.D.
    Characterisation of recombinant human fatty aldehyde dehydrogenase: implications for Sjoegren-Larsson syndrome (2007), J. Enzyme Inhib. Med. Chem., 22, 584-590.
    View publication on PubMed
Search for more literature for 1.2.1.48

Application

Application Comment Organism
medicine direct link between FALDH inactivation and disease pathology of Sjögren-Larsson syndrome caused by accumulation of alcohols Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant His6-FADH expressed in Escherichia coli BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
D245N NAD+ cosubstrate binding is occuring but catalytic reduction is diminished Homo sapiens
G185A disruption of NAD+ binding/utilisation Homo sapiens
G412R disruption of interaction of side-chains with substrate Homo sapiens
H411Y disruption of the key abstraction of the hydroxyl hydrogen Homo sapiens
Q445R substitution in the recombinant protein Homo sapiens
T184M disruption of NAD+ binding/utilisation Homo sapiens
T184R disruption of NAD+ binding/utilisation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
 decanal
-
 Homo sapiens
0.0083
-
 hexadecanal
-
 Homo sapiens
0.0103
-
 Tetradecanal
-
 Homo sapiens
0.0136
-
 dodecanal
-
 Homo sapiens
0.02
-
 octadecanal
-
 Homo sapiens
0.023
-
 farnesal
-
 Homo sapiens
0.0343
-
 (11Z)-hexadec-11-enal
-
 Homo sapiens
0.18
-
 NAD+
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-FADH purified by anion exchange and metal-chelate chromatography, to 90-95% purity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-11-hexadecenal + NAD+ + H2O
-
 Homo sapiens cis-11-hexadecenoic acid + NADH + H+
-
 ?
decanal + NAD+ + H2O
-
 Homo sapiens decanoic acid + NADH + H+
-
 ?
dodecanal + NAD+ + H2O
-
 Homo sapiens dodecanoic acid + NADH + H+
-
 ?
farnesal + NAD+ + H2O
-
 Homo sapiens farnesoic acid + NADH + H+
-
 ?
heptanal + NAD+ + H2O
-
 Homo sapiens heptanoic acid + NADH + H+
-
 ?
hexadecanal + NAD+ + H2O
-
 Homo sapiens hexadecanoic acid + NADH + H+
-
 ?
additional information catalytically important residues to be involved in alcohol and aldehyde oxidation are Gln-120, Glu-207, Cys-241, Phe-333, Tyr-410 and His-411 Homo sapiens ?
-
 ?
octadecanal + NAD+ + H2O
-
 Homo sapiens octadecanoic acid + NADH + H+
-
 ?
octanal + NAD+ + H2O
-
 Homo sapiens octanoic acid + NADH + H+
-
 ?
tetradecanal + NAD+ + H2O
-
 Homo sapiens tetradecanoic acid + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
FALDH
-
 Homo sapiens
fatty aldehyde dehydrogenase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.86
-
 Tetradecanal
-
 Homo sapiens
0.93
-
 farnesal
-
 Homo sapiens
0.95
-
 hexadecanal
-
 Homo sapiens
1.28
-
 NAD+
-
 Homo sapiens
1.4
-
 (11Z)-hexadec-11-enal
-
 Homo sapiens
1.52
-
 octadecanal
-
 Homo sapiens
2.18
-
 decanal
-
 Homo sapiens
2.23
-
 dodecanal
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens