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Literature summary for 1.2.1.5 extracted from

  • Perozich, J.; Kuo, I.; Wang, B.C.; Boesch, J.S.; Hempel, J.
    Shifting the NAD/NADP preference in class 3 aldehyde dehydrogenase (2000), Eur. J. Biochem., 267, 6197-6203.
    View publication on PubMed
Search for more literature for 1.2.1.5

Protein Variants

Protein Variants Comment Organism
E140D Km-value for NADP+ is about half that of the native enzyme, Km-value for NAD+ is about 3.1fold that of the native enzyme, Km-value for benzaldehyde is about 1.4fold that of the native enzyme, Km-value for propanal is about 1.9fold that of the native enzyme. The coenzyme preference NADP+/NAD+ is 3.2, compared to 3.4 for the native enzyme Rattus norvegicus
E140N Km-value for NADP+ is about 10% of that of the native enzyme, Km-value for NAD+ is about 3.2fold that of the native enzyme, Km-value for benzaldehyde is about 1.2fold that of the native enzyme, Km-value for propanal is about 2.3fold that of the native enzyme. The coenzyme preference NADP+/NAD+ is 4.2, compared to 3.4 for the native enzyme Rattus norvegicus
E140Q Km-value for NADP+ is about 10% of that of the native enzyme, Km-value for NAD+ is about 7.9fold that of the native enzyme, Km-value for benzaldehyde is about 1.6fold that of the native enzyme, Km-value for propanal is about 1.1fold that of the native enzyme. The coenzyme preference NADP+/NAD+ is 2.8, compared to 3.4 for the native enzyme Rattus norvegicus
E140T Km-value for NADP+ is about 20% of that of the native enzyme, Km-value for NAD+ is about 15.3fold that of the native enzyme, Km-value for benzaldehyde is about 60% of that of the native enzyme, Km-value for propanal is about 1.4fold that of the native enzyme. The coenzyme preference NADP+/NAD+ is 5.8, compared to 3.4 for the native enzyme Rattus norvegicus
G192A Km-value for NADP+ is about half that of the native enzyme, Km-value for NAD+ is about 70% of that of the native enzyme, Km-value for benzaldehyde is about 90% of that of the native enzyme, Km-value for propanal is about 45% of that of the native enzyme Rattus norvegicus
K137Q Km-value for NADP+ is about 2.4fold that of the native enzyme, Km-value for NAD+ is about 16fold that of the native enzyme, Km-value for benzaldehyde is about 1.2fold that of the native enzyme, Km-value for propanal is about 95% of that of the native enzyme. The coenzyme preference NADP+/NAD+ is 1.1, compared to 3.4 for the native enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
NADH
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + NADP+
-
 Rattus norvegicus benzoate + NADPH
-
 ?
propanal + NAD+ + H2O
-
 Rattus norvegicus propionate + NADH + H+
-
 ?