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Literature summary for 1.2.1.5 extracted from

  • Wang, B.; Chen, X.; Wang, Z.; Xiong, W.; Xu, T.; Zhao, X.; Cao, Y.; Guo, Y.; Li, L.; Chen, S.; Huang, S.; Wang, X.; Fang, M.; Shen, Z.
    Aldehyde dehydrogenase 1A1 increases NADH levels and promotes tumor growth via glutathione/dihydrolipoic acid-dependent NAD+ reduction (2017), Oncotarget, 8, 67043-67055 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.5

Protein Variants

Protein Variants Comment Organism
additional information simultaneous knockout of ALDH1A1 (EC 1.2.1.36) and its isozyme ALDH3A1 in lung cancer cell line NCI-H460 inhibits tumor growth in a xenograft model. Moreover, the ALDH1A1 mutants that retain their GSH/DHLA-dependent NAD+ reduction activity and lose their aldehyde-dehydrogenase activity are able to decrease the NAD+/NADH ratio and to rescue the impaired growth of ALDH1A1/3A1 double knockout tumor cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P30838
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Purification (Commentary)

Purification (Comment) Organism
purification of the enzyme(s) responsible for the GSH/DHLA-dependent NAD+ reduction activity from large-scale cell cultures, native enzyme from EKVX lung cancer cells by ultracentrifugation of crude cell extract and anion echange chromatography, hydrophobic interaction and hydroxyapatite chromatography, followed by gel filtration and another different step of anion exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
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 Homo sapiens
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EKVX cell
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 Homo sapiens
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lung cancer cell
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 Homo sapiens
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additional information ALDH3A1 is highly expressed in lung carcinoma cell line A549 and EKVX, as well as in the large cell carcinoma cell line H460. Isozyme ALDH1A1 (EC 1.2.1.36) shows a similar expression pattern like ALDH3A1. Other members of the aldehyde dehydrogenase family are not expressed in the lung cancer cell lines examined Homo sapiens
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NCI-H460 cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glutathione + NAD+
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 Homo sapiens GSSG + NADH + H+
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 ?
dihydrolipoic acid + NAD+
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 Homo sapiens alpha-lipoic acid + NADH + H+
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 ?
additional information ALDH1A1 can also use glutathione (GSH) and dihydrolipoic acid (DHLA) as electron donors to reduce NAD+ to NADH, product identification by thin layer chromatography (TLC) Homo sapiens ?
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Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase 3A1
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 Homo sapiens
ALDH3A1
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 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
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 Homo sapiens

General Information

General Information Comment Organism
evolution aldehyde dehydrogenase 1A1 (ALDH3A1) is a member of the aldehyde dehydrogenase superfamily that oxidizes aldehydes to their corresponding acids, reactions that are coupled to the reduction of NAD(P)+ to NAD(P)H Homo sapiens
metabolism ALDH1A1 and ALDH3A1 have the same GSH/DHLA-dependent NAD+-reduction activity Homo sapiens
physiological function aldehyde dehydrogenase 3A1 increases NADH levels and promotes tumor growth via glutathione/dihydrolipoic acid-dependent NAD+ reduction. ALDH3A1 can also use glutathione (GSH) and dihydrolipoic acid (DHLA) as electron donors to reduce NAD+ to NADH. The GSH/DHLA-dependent NAD+-reduction activity of ALDH1A1 is not affected by the aldehyde dehydrogenase inhibitor or by mutation of the residues in its aldehyde-binding pocket. It is thus a distinct biochemical reaction from the classic aldehyde-dehydrogenase activity catalyzed by ALDH3A1. The GSH/DHLA-dependent NAD+-reduction activity of ALDH3A1 can decrease cellular NAD+/NADH ratio and promote tumor growth Homo sapiens