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Literature summary for 1.2.1.57 extracted from
- Purification and properties of the inducible coenzyme A-linked butyraldehyde dehydrogenase from Clostridium acetobutylicum (1988), J. Bacteriol., 170, 2971-2976.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol or dithiothreitol helps to maintain enzyme activity | Clostridium acetobutylicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoASH | at 0.3 mM | Clostridium acetobutylicum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
NADH | - |
Clostridium acetobutylicum | |
| 0.04 | - |
NADPH | - |
Clostridium acetobutylicum | |
| 0.045 | - |
butanoyl-CoA | CoA | Clostridium acetobutylicum | |
| 0.055 | - |
acetyl-CoA | - |
Clostridium acetobutylicum | |
| 0.16 | - |
NAD+ | - |
Clostridium acetobutylicum | |
| 0.37 | - |
hexanal | - |
Clostridium acetobutylicum | |
| 1.6 | - |
Butanal | - |
Clostridium acetobutylicum | |
| 2 | - |
NADP+ | - |
Clostridium acetobutylicum | |
| 6.7 | - |
propionaldehyde | - |
Clostridium acetobutylicum | |
| 7.2 | - |
acetaldehyde | - |
Clostridium acetobutylicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000, SDS-PAGE | Clostridium acetobutylicum |
| 115000 | - |
gel filtration | Clostridium acetobutylicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.1 | - |
- |
Clostridium acetobutylicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, half-life: 3 days, enzyme in crude extract | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + CoA + NAD+ | - |
Clostridium acetobutylicum | acetyl-CoA + NADH | - |
r | |
| butanal + CoA + NAD+ | r | Clostridium acetobutylicum | butanoyl-CoA + NADH | - |
r | |
| butanal + CoA + NAD+ | affinity for NAD+ or NADH is greater than for NADP+ or NADPH | Clostridium acetobutylicum | butanoyl-CoA + NADH | - |
r | |
| butanal + CoA + NADP+ | r | Clostridium acetobutylicum | butanoyl-CoA + NADPH | - |
r | |
| butanal + CoA + NADP+ | affinity for NAD+ or NADH is greater than for NADP+ or NADPH | Clostridium acetobutylicum | butanoyl-CoA + NADPH | - |
r | |
| hexanal + CoA + NAD+ | - |
Clostridium acetobutylicum | hexanoyl-CoA + NADH | - |
r | |
| propionaldehyde + NAD+ | - |
Clostridium acetobutylicum | propanoyl-CoA + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 56000, SDS-PAGE | Clostridium acetobutylicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
formation of butyraldehyde | Clostridium acetobutylicum |
| 9 | - |
formation of butyryl-CoA | Clostridium acetobutylicum |
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