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Literature summary for 1.2.1.59 extracted from

  • Ito, F.; Chishiki, H.; Fushinobu, S.; Wakagi, T.
    Comparative analysis of two glyceraldehyde-3-phosphate dehydrogenases from a thermoacidophilic archaeon, Sulfolobus tokodaii (2012), FEBS Lett., 586, 3097-3103.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no influence on activity is observed on assaying of the enzyme with 5 mM substrate and 0.5 mM co-substrate with 1 mM ATP, ADP, AMP, glucose 6-phosphate, fructose 6-phosphate, galactose 1-phosphate, glucose 1-phosphate or coenzyme A in either the glycolytic or gluconeogenic reaction Sulfurisphaera tokodaii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
3-phospho-D-glyceroyl phosphate
-
Sulfurisphaera tokodaii
D-glyceraldehyde 3-phosphate
-
Sulfurisphaera tokodaii
additional information no influence on activity is observed on assaying of the enzyme with 5 mM substrate and 0.5 mM co-substrate with 1 mM ATP, ADP, AMP, glucose 6-phosphate, fructose 6-phosphate, galactose 1-phosphate, glucose 1-phosphate or coenzyme A in either the glycolytic or gluconeogenic reaction Sulfurisphaera tokodaii
NAD+
-
Sulfurisphaera tokodaii
NADH
-
Sulfurisphaera tokodaii
NADP+
-
Sulfurisphaera tokodaii
NADPH
-
Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
NADPH pH 8.0, 60°C Sulfurisphaera tokodaii
0.21
-
D-glyceraldehyde 3-phosphate pH 8.0, 60°C, cosubstrate: NADP+ Sulfurisphaera tokodaii
0.28
-
NADP+ pH 8.0, 60°C Sulfurisphaera tokodaii
0.77
-
D-glyceraldehyde 3-phosphate pH 8.0, 60°C, cosubstrate: NAD+ Sulfurisphaera tokodaii
5.8
-
3-phospho-D-glyceroyl phosphate pH 8.0, 60°C Sulfurisphaera tokodaii
16.8
-
NAD+ pH 8.0, 60°C Sulfurisphaera tokodaii
107
-
phosphate pH 8.0, 60°C Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADPH + H+ Sulfurisphaera tokodaii in the gluconeogenic direction the enzyme is specific for NADPH D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
r
3-phospho-D-glyceroyl phosphate + NADPH + H+ Sulfurisphaera tokodaii 7 in the gluconeogenic direction the enzyme is specific for NADPH D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Sulfurisphaera tokodaii both NAD+ and NADP+ are utilized in the glycolytic direction 3-phospho-D-glyceroyl phosphate + NADH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Sulfurisphaera tokodaii 7 both NAD+ and NADP+ are utilized in the glycolytic direction 3-phospho-D-glyceroyl phosphate + NADH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Sulfurisphaera tokodaii both NAD+ and NADP+ are utilized in the glycolytic direction 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Sulfurisphaera tokodaii 7 both NAD+ and NADP+ are utilized in the glycolytic direction 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q971K2
-
-
Sulfurisphaera tokodaii 7 Q971K2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glyceroyl phosphate + NADPH + H+ in the gluconeogenic direction the enzyme is specific for NADPH Sulfurisphaera tokodaii D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
r
3-phospho-D-glyceroyl phosphate + NADPH + H+ in the gluconeogenic direction the enzyme is specific for NADPH Sulfurisphaera tokodaii 7 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+ both NAD+ and NADP+ are utilized in the glycolytic direction Sulfurisphaera tokodaii 3-phospho-D-glyceroyl phosphate + NADH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+ comparable activity with NADP+ and NAD+ Sulfurisphaera tokodaii 3-phospho-D-glyceroyl phosphate + NADH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+ both NAD+ and NADP+ are utilized in the glycolytic direction Sulfurisphaera tokodaii 7 3-phospho-D-glyceroyl phosphate + NADH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NAD+ comparable activity with NADP+ and NAD+ Sulfurisphaera tokodaii 7 3-phospho-D-glyceroyl phosphate + NADH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+ both NAD+ and NADP+ are utilized in the glycolytic direction Sulfurisphaera tokodaii 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+ comparable activity with NADP+ and NAD+ Sulfurisphaera tokodaii 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+ both NAD+ and NADP+ are utilized in the glycolytic direction Sulfurisphaera tokodaii 7 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+ comparable activity with NADP+ and NAD+ Sulfurisphaera tokodaii 7 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
GAPDH
-
Sulfurisphaera tokodaii
STK_13560 locus name Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NAD+ comparable activity with NADP+ and NAD+ Sulfurisphaera tokodaii
NADP+ comparable activity with NADP+ and NAD+ Sulfurisphaera tokodaii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.035
-
NADPH pH 8.0, 60°C Sulfurisphaera tokodaii
0.081
-
NADPH pH 8.0, 60°C Sulfurisphaera tokodaii
0.75
-
NADP+ pH 8.0, 60°C Sulfurisphaera tokodaii
2.8
-
D-glyceraldehyde 3-phosphate pH 8.0, 60°C, cosubstrate: NAD+ Sulfurisphaera tokodaii
10.7
-
NAD+ pH 8.0, 60°C Sulfurisphaera tokodaii
13.1
-
D-glyceraldehyde 3-phosphate pH 8.0, 60°C, cosubstrate: NADP+ Sulfurisphaera tokodaii
49.1
-
3-phospho-D-glyceroyl phosphate pH 8.0, 60°C Sulfurisphaera tokodaii