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Literature summary for 1.2.1.59 extracted from

  • Tian, L.; Perot, S.; Stevenson, D.; Jacobson, T.; Lanahan, A.; Amador-Noguez, D.; Olson, D.; Lynd, L.
    Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol (2017), Biotechnol. Biofuels, 10, 276 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.59

Cloned(Commentary)

Cloned (Comment) Organism
gene gapdh, recombinant expression of the Thermoanaerobacterium saccharolyticum Gapdh in Clostridium thermocellum Thermoanaerobacterium saccharolyticum

Inhibitors

Inhibitors Comment Organism Structure
additional information a dramatic accumulation of NADH and NADPH is observed when ethanol is added to the culture. The Gapdh from Thermoanaerobacterium saccharolyticum is less sensitive to the NADH/NAD+ ratio Thermoanaerobacterium saccharolyticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ Thermoanaerobacterium saccharolyticum
-
 3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ Thermoanaerobacterium saccharolyticum DSM 8691
-
 3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ Thermoanaerobacterium saccharolyticum JW/SL-YS485
-
 3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium saccharolyticum I3VY89
-
-
Thermoanaerobacterium saccharolyticum DSM 8691 I3VY89
-
-
Thermoanaerobacterium saccharolyticum JW/SL-YS485 I3VY89
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
-
 Thermoanaerobacterium saccharolyticum 3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
-
 Thermoanaerobacterium saccharolyticum DSM 8691 3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+
-
 Thermoanaerobacterium saccharolyticum JW/SL-YS485 3-phospho-D-glyceroyl phosphate + NAD(P)H + H+
-
 ?

Synonyms

Synonyms Comment Organism
glyceraldehyde 3-phosphate dehydrogenase
-
 Thermoanaerobacterium saccharolyticum
Tsac_2486
-
 Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Thermoanaerobacterium saccharolyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Thermoanaerobacterium saccharolyticum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermoanaerobacterium saccharolyticum
NADP+
-
 Thermoanaerobacterium saccharolyticum

General Information

General Information Comment Organism
additional information comparison of Gapdh protein from Clostridium thermocellum and Thermoanaerobacterium saccharolyticum, homology modeling, overview. The Gapdh from Thermoanaerobacterium saccharolyticum is less sensitive to ethanol and the NAD+/NADH ratio. Recombinant Gapdh from Thermoanaerobacterium saccharolyticum expressed in Clostridium thermocellum cells can improve the growth rate and ethanol resistance Thermoanaerobacterium saccharolyticum