Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | competitive inhibition with respect to NAD+, Ki: 0.027 mM | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
5-carboxymethyl-2-hydroxymuconate semialdehyde | - |
Pseudomonas putida | |
0.02 | - |
NAD+ | - |
Pseudomonas putida | |
0.048 | - |
NAD+ | - |
Pseudomonas putida | |
1.2 | - |
NADP+ | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52500 | - |
3 * 52500, SDS-PAGE | Pseudomonas putida |
200000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Pseudomonas putida | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
frozen, 12 months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Pseudomonas putida | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+ | 18% of the activity compared to reduction with NAD+ | Pseudomonas putida | 5-carboxymethyl-2-hydroxymuconate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 3 * 52500, SDS-PAGE | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable for 30 min | Pseudomonas putida |
47 | - |
complete loss of activity after 20 min | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
5-carboxymethyl-2-hydroxymuconate semialdehyde | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strict dependency for NAD+ reduction | Pseudomonas putida | |
NADP+ | 82% lower activity compared to NAD+ | Pseudomonas putida |