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Literature summary for 1.2.1.61 extracted from

  • Kolvenbach, B.A.; Dobrowinski, H.; Fousek, J.; Vlcek, C.; Schaeffer, A.; Gabriel, F.L.; Kohler, H.P.; Corvini, P.F.
    An unexpected gene cluster for downstream degradation of alkylphenols in Sphingomonas sp. strain TTNP3 (2012), Appl. Microbiol. Biotechnol., 93, 1315-1324.
    View publication on PubMed
Search for more literature for 1.2.1.61

Cloned(Commentary)

Cloned (Comment) Organism
HqdC expression in Escherichia coli BL21-DE3 cells Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53500
-
 calculated from sequence Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. F8TW85
-
-
Sphingomonas sp. TTNP3 F8TW85
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0006
-
 uninduced cells, pH 7.0, temperature not specified in the publication Sphingomonas sp.
0.0025
-
 hydroquinone-induced cells, pH 7.0, temperature not specified in the publication Sphingomonas sp.
0.0031
-
 nonylphenol-induced cells, pH 7.0, temperature not specified in the publication Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxymuconic semialdehyde + NAD+ + H2O
-
 Sphingomonas sp. maleylacetate + NADH + 2 H+
-
 ?
4-hydroxymuconic semialdehyde + NAD+ + H2O
-
 Sphingomonas sp. TTNP3 maleylacetate + NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
4-hydroxymuconic semialdehyde dehydrogenase
-
 Sphingomonas sp.
HqdC
-
 Sphingomonas sp.

Expression

Organism Comment Expression
Sphingomonas sp. by 0.5 mM hydroquinone and by 0.5 mM nonylphenol up

General Information

General Information Comment Organism
metabolism HqdC gene is part of a gene cluster associated with hydroquinone degradation into 3-oxoadipate Sphingomonas sp.